灯盏细辛的抗氧化活性研究  

Antioxidant Activity of Constituents from Erigeron breviscapus

作　　者：党翠芝[1] 黄小燕[1] 杨庆雄[2] 艾俊丽[1] 田猛[1] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001 [2]贵州师范大学化学与材料科学学院,贵阳550001

出　　处：《中国实验方剂学杂志》2012年第17期100-103,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省国际科技合作计划项目(黔科合外G字[2010]7023);贵州师范大学学生科研基金

摘　　要：目的:研究灯盏细辛6个化合物的抗氧化活性。方法:以灯盏细辛为原料,采用有机溶剂提取法和色谱柱法对灯盏细辛中化学成分进行提取与分离,利用核磁共振等波谱学方法进行结构鉴定,体外法检测6个化合物对1-二苯基-2-苦味酰肼自由基清除法(DPPH),2,2'-联氨-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除法(ABTS),O2-.自由基和.OH自由基的清除能力,并以维生素C(Vc)为阳性对照,结果:6个化合物者对DPPH,ABTS,O2-.,.OH自由基均具有清除作用,且与浓度呈良好的量效关系,6个化合物清除自由基能力与Vc相似。结论:灯盏细辛中的6个化合物均具有较强的抗氧化活性。Objective： To study the antioxidant activity of 6 compounds isolated from Erigeron breviscapus. Method： Compounds were isolated and acquired from E. breviscapus through the methods of organic solvent extraction and chromatography, and identified by the spectrum of NMR （nuclear magnetic resonance）. The scavenging activities of all these compounds against 1, 1-diphenyl-2-picrylhydrazyl radical （DPPH） radicals, 2, 2＇-amino-di （2-ethyl-benzothiazoline sulphonic acid-6 ） ammonium salt （ABTS） radicals, O2· and ·OH radicals were evaluated by in vitro method and compared with Vc as positive control. Result ： All the six compounds have scavenging capability against DPPH radicals, ABTS radicals, O2- ·and -OH radicals and have a good doseresponses relationship with the concentration. The differences between the free radical scavenging capacity of six compounds and Vc are were significant. Conclusion： Six pounds isolated from E. breviscapus have the stronger antioxidant activity.

关 键 词：灯盏细辛 自由基 清除能力 抗氧化活性 

分 类 号：R285.5[医药卫生—中药学]