凹叶厚朴不同组织器官挥发性物质比较分析  

Evaluation of Volatiles of the Different Tissues from Magnolia officinalis

作　　者：杨占南[1] 罗世琼[2] 余正文[2] 胡光平[1,2] 王帆[1,2] 周欣[1] 赵超[1] 

机构地区：[1]贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵阳550001 [2]贵州师范大学生命科学院,贵阳550001

出　　处：《中国实验方剂学杂志》2012年第17期115-119,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(No.31060056);贵州省科学技术联合基金项目(黔J字LKS[2009]23号);贵州省科技创新人才团队建设项目[黔科合人才团队(2011)4008]

摘　　要：目的:评价不同组织器官的挥发性成分的累积分布。方法:利用顶空固相微萃取(HS-HPME)结合气相色谱-质谱联用(GC-MS)对凹叶厚朴不同组织器官(叶芽、根皮、茎皮、枝皮、叶、果皮和种子)的挥发性成分进行分析。结果:这些组织的挥发性成分主要是α-芳樟醇0.21%～4.92%、α-石竹烯7.48%～38.71%、β-石竹烯1.53%～14.43%、4(14),11-桉叶二烯0.15%～10.02%、β-花柏烯0.56%～10.68%、石竹烯氧化物1.68%～36.01%、α-桉叶醇1.56%～33.79%和β-桉叶醇0.19%～28.89%,精油产率为0.12%～0.96%。结论:植株的叶芽、根皮、茎皮、枝皮、叶、果皮和种子挥发精油的产率、挥发性成分以及这些成分的累积含量差异显著。Objective： To evaluate accumulation of the volatiles of the different tissues in Magnolia officinalis. Method： The volatiles of leaf buds, root skins, barks, twig skins, leaves, pericarps and seeds of M. officinalis were analyzed using headspace solid phase micro-extraction （HS-SPME） following by capillary gas chromatography coupled to mass spectrometry （GC-MS）. Result： The volatiles were α-linalool 0.21% -4.92% ,α- caryophyllene 7.48%-38.71% , fl-caryophyllene 1.53%-14.43% , eudesma-4 （ 14 ） , ll-diene 0.15%- 10.02% , β-chamigrene 0.56% -10.68% , caryophyllene oxide 1.68% -36.01% , α-eudesmol 1.56% -33.79% and β-eudesmol 0. 19%-28.89%. The yields of essential oils in the different tissues were 0.12%-0. 96%. Conclusion： The essential oil, the volatiles composition and their accumulation content of leaf buds, root skins, barks, twig skins, leaves, pericarps and seeds of M. officinalis were significantly different.

关 键 词：凹叶厚朴 组织器官 挥发性物质 评价 

分 类 号：R284.1[医药卫生—中药学]