三清降糖方对α-葡萄糖苷酶抑制作用的研究  

Study on α-glucosidase Inhibitory Effect of Sanqing Jiangtang Fang

作　　者：高秀娟[1] 吴范武[1] 江春花[1] 李继安[1] 喇孝瑾[1] 

机构地区：[1]河北联合大学中医学院,河北唐山063000

出　　处：《中国实验方剂学杂志》2012年第17期247-249,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：唐山市科技局攻关项目(10140201A-18)

摘　　要：目的:观察三清降糖方对α-葡萄糖苷酶活性的影响。方法:提取大鼠小肠的α-葡萄糖苷酶,测定其含量,通过正交试验确定最佳反应体系,制备三清降糖方水提制剂和醇提制剂,分别配制成25,2.5,0.25,0.025,0.002 5 g.L-1的溶液,以阿卡波糖为阳性药对照组,用葡萄糖氧化酶法测定葡萄糖含量,计算抑制率。结果:三清降糖方水提制剂、醇提制剂对α-葡萄糖苷酶活性的抑制有明显的浓度-效应关系。质量浓度为25 g.L-1三清降糖方水提制剂对α-葡萄糖苷酶活性的抑制率为(64.85±10.5)%,与2.5 g.L-1的阿卡波糖抑制率相当。结论:三清降糖方具有抑制α-葡萄糖苷酶活性的作用。Objective： To observe the influence of Sanqing Jiangtang Fang on α-glucosidase activity. Method： α-glucosidase from small intestine of rats was extracted and the content was determined. The optimal reaction system was set by orthogonal experiment. Sanqing Jiangtang Fang was extracted with water and ethanol respectively and prepared into solutions at 25, 2.5, 0.25, 0. 025, 0. 002 5 g ·L-1. Acarbose was used as the positive drug. The content of glucose was measured by glucose oxidase method to evaluate the inhibitory effect on α- glucosidase. The inhibitory rate was calculated. Result： Water-extraction preparation and ethanol-extraction preparation of Sanqing Jiangtang Fang could inhibit α-glucosidase activity, and showed marked concentration-effect relationship. The inhibitory effect of water-extraction preparation at 25 g·L-1 was 64.85% ± 10.5% , which was in correspondence to acarbose at 2.5 g ·L-1. Conclusion： Sanqing Jiangtang Fang can inhibit α-glucosidase activity.

关 键 词：三清降糖方 水提制剂 醇提制剂 Α-葡萄糖苷酶 

分 类 号：R285.5[医药卫生—中药学]