静脉输注高氧液对家兔小肠缺血再灌注损伤的影响  

Effect of intravenous infusion of hyper-oxygenated solution on small intestinal ischemia-reperfusion injury in rabbits

作　　者：龚辉 高昌俊[2] 郭瑜[2] 牛江涛[2] 姜娜 卢江明 吴波 

机构地区：[1]陕西省妇幼保健院麻醉科,西安市710003 [2]第四军医大学唐都医院麻醉科

出　　处：《中华麻醉学杂志》2012年第7期874-876,共3页Chinese Journal of Anesthesiology

基　　金：陕西省科技攻关项目（2010k15-07）

摘　　要：目的评价静脉输注高氧液对家兔小肠缺血再灌注损伤的影响。方法健康成年家兔24只，雌雄不拘，体重2．5～3．2kg，采用随机数字表法，将家兔随机分为3组（n=8）：假手术组（S组）、小肠缺血再灌注组（I／R组）和静脉输注高氧液组（HOS组）。S组仅开腹游离但不夹闭肠系膜上动脉，I／R组和I-IOS组采用夹闭肠系膜上动脉1h恢复灌注2h的方法制备家兔小肠缺血再灌注模型，于夹闭肠系膜上动脉即刻HOS组耳缘静脉输注高氧液20ml·kg-1·h-1，I／R组静脉输注等容量生理盐水。于再灌注2h时采集下腔静脉血样，检测血清乳酸浓度，处死家兔取小肠组织，测定丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧物酶（GSH-Px）的活性，光镜及电镜下观察肠上皮组织病理学结果，行肠黏膜损伤评分。取内脏组织，观察细菌移位情况。结果与S组相比，I／R组和HOS组肠组织MDA含量、血清乳酸浓度和细菌移位率升高，SOD、CAT和GSH—Px活性降低（P〈0．05）；与I／R组相比，HOS组肠组织MDA含量、血清乳酸浓度和细菌移位率降低，SOD、CAT和GSH—Px活性降低（P〈0．05）。结论静脉输注高氧液可减轻家兔小肠缺血再灌注损伤。Objective To investigate the effect of intravenous infusion of hyper-oxygenated solution （HOS） on small intestinal isehemia-reperfusion （I/R） injury in rabbits. Methods Twenty-four rabbits of both sexes, weighing 2.5-3.2 kg, were randomly divided into 3 groups （n = 8 each） ： sham operation group （group S）, I/R group, and HOS group. Small intestinal I/R was produced by clamping the superior mesenteric artery （SMA） for 1 h followed by 2 h of reperfusion in I/R and HOS groups, while the SMA was only clamped in group S. HOS was infused intravenously at a rate of 20 ml·kg-1·h-1 via the auricular vein starting from the time immediately after clamping the SMA in group HOS and the equal volume of normal saline was infused instead of HOS in group I/ R. Blood samples were obtained from the inferior vena cava at 2 h of reperfusion to detect the concentration of serum lactic acid. The animals were then sacrificed and the small intestine was removed for determination of the malondialdehyde （MDA） content, and activities of superoxide dismutase （SOD）, catalase （CAT） and glntathione peroxidase （GSH-Px） in intestinal tissues and for microscopic examination. The pathological changes of the intestinal epithelia were observed and the damage to the mucous membrane was scored. The internal organs were removed and bacterial translocation from gut to the internal organs was observed. Results Compared with group S, the level of MDA and lactic acid, and rate of bacterial transloeation were significantly increased, and the activities of SOD, CAT and GSH-Px were significantly decreased in groups I/R and HOS （ P 〈 0.05）. Compared with group I/R, the level of MDA and lactic acid, rate of bacterial translocation, and activities of SOD, CAT and GSH-Px were significantly decreased in group HOS （ P 〈 0.05）. Conclusion Intravenous infusion of HOS can reduce small intestinal I/R injury in rabbits.

关 键 词：氧 输注 静脉内 再灌注损伤 小肠 

分 类 号：R363[医药卫生—病理学]