采用离子对试剂同时测定啤酒中异α-酸和还原异α-酸  

Simultaneous determination of iso-alpha-acid and reduced iso-alpha-acids in beer using ion pair reagent by HPLC

作　　者：杨朝霞 黄淑霞 田玉红 郝俊光 尹花 

机构地区：[1]啤酒生物发酵工程国家重点实验室(筹),青岛啤酒股份有限公司,青岛266061

出　　处：《分析试验室》2012年第9期30-33,共4页Chinese Journal of Analysis Laboratory

基　　金：国家973项目(2010CB735706)资助

摘　　要：建立了使用离子对试剂同时检测啤酒中异α-酸、二氢异α-酸、四氢异α-酸和六氢异α-酸的反相高效液相色谱法。实验采用四丁基氢氧化铵-磷酸缓冲液(pH调至7.2)-乙腈为流动相,Xterra RP C18(4.6×150 mm,5μm)色谱柱,等度洗脱,实现这4种异构化α-酸的所有组分的有效分离测定。各组分色谱峰保留时间的RSD为0.1%～0.3%,峰面积的RSD为0.5%～1.7%(n=7),重现性和线性关系较好;4种异构化α-酸的加标回收率为96%～108%。该方法无需样品处理,简便易行,可用于分析啤酒中所含异构化α-酸的种类和含量。A method for the simultaneous determination of iso-alpha-acid, dihydro-iso-alpha-acid, tetrahydro- iso-aJpha-acid and hexahydro-iso-alpha-aeid in beer using ion pair reagent by reversed phase high performance liquid chromatography is described. The assay was conducted on a Xterra RP Cls （4. 6 x 150 ram,5 pLm）column using isometric elution with a tetrabutylammonium hydroxide-phosphoric acid buffer at a pH of 7.2. All of components were separated completely. Good linear relationship and reproducibility were obtained. RSDs were 0. 1% -0. 3% and 0. 5% - 1.7% for retention time and peak areas, respectively. The average recoveries were 96% -108%. The method was operated simply without sample pretreatment, and it can be used to analyze the types and contents of iso-alpha-acid and reduced iso-alpha-acids in beer.

关 键 词：离子对试剂 反相高效液相色谱 啤酒 异构化α-酸 还原异构化α-酸 检测 

分 类 号：O657.5[理学—分析化学]