伴和不伴癌的乳腺导管内增生性病变ERα与p53蛋白表达差异的研究  

p53 nuclear accumulation and ERα expression in ductal hyperplasia co-existing with breast cancer versus pure ductal hyperplasia without breast cancer

作　　者：毛晓韵[1] 陈浩[1] 王欢[1] 魏晶[1] 范垂锋[2] 姚凡[1] 刘崇[1] 金锋[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,普通外科教研室,乳腺外科,辽宁沈阳110001 [2]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2012年第13期961-965,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30950009)

摘　　要：目的:研究伴癌的乳腺导管内增生性病变与不伴癌的乳腺导管内增生性病变ERα与p53蛋白表达的差异。方法:用免疫组化法研究129例不伴癌的乳腺导管内增生性病变及86例伴癌的乳腺导管内增生性病变中p53及ERα蛋白表达差异。结果:p53蛋白阳性表达于18.2%(14/77)单纯性非典型导管增生(ADH),31.0%(9/29)伴原位癌的ADH(ADH/DCIS),26.7%(8/30)伴浸润性导管癌的ADH(ADH/IDC),三者间表达率差异无统计学意义,P>0.05。ERα在79.3%(23/29)伴原位癌的ADH(ADH/DCIS)中阳性表达,在76.7%(23/30)的伴浸润性导管癌的ADH(ADH/IDC)中阳性表达,均明显低于其在单纯性ADH中的表达(72/77,93.5%),P<0.05。在乳腺导管内增生性病变中,突变型p53蛋白的核聚集与ERα阳性表达的相关系数r=-0.512,P<0.01。结论:初步研究结果提示,虽然不伴癌的ADH与伴癌的ADH有相同的形态学表现,但形成的分子机制可能存在差异。OBJECTIVE： To discuss the expression of p53 and ERa in ductal hyperplasia co-existing with ductal carcinoma in situ （DCIS） or invasive ductal carcinoma （IDC） versus pure ductal hyperplasia without DCIS or IDC. METH- ODS： We investigated p53 nuclear accumulation and ERa expression in breast ductal hyperplasia in a cohort of 215 chinese women by immunohistochemistry （IHC）, which included 129 cases of pure ductal hyperplasia, 86 cases of duetal hyper- plasia co-existing with DCIS （41 cases） or IDC （45 cases）. RESULTS： No difference in nuclear p53 accumulation was ob- served between pure ADH （18.2%,14/77） and ADH co existing with DCIS （ADH/DCIS,31.0%,9/29） or IDC（ADH/ IDC,26.7%,8/30,P〈0.05）. The frequency of ERa expression was lower in ADH/DCIS （23/29, 79.3%） and ADH/ IDC （23/30, 76.7%） than that in pure ADH （72/77, 93.5%） respectively （P〈0.05）. There was a negative weak cor- relation between p53 nuclear accumulation and ERa expression as for ADH （coefficient correlation -0.51, P〈0. 001）. CONCLUSIONS： At least some pure ADH is molecularly distinct from ADH/DCIS or ADH/IDC which indicated the two types of ADH are molecularly distinct entities although they have the same morphological appearance.

关 键 词：乳腺导管内增生性病变 免疫组织化学 P53 雌激素受体Α 

分 类 号：R737.9[医药卫生—肿瘤]