含噻唑烷-4-酮的糖类衍生小分子免疫调节剂对人淋巴细胞分化的影响  

Effects of C-pseudonucleosides bearing thiazolidin-4-one as on differentiations of human lymphocytes

作　　者：孟明[1] 李春晓[1] 洪洋[1] 张金库[2] 刘红梅[3] 陈冬志[1] 陈华[1] 李小六[1] 

机构地区：[1]河北大学医学部,保定071000 [2]保定市第一中心医院 [3]教育部药物化学与分子诊断省部共建重点实验室

出　　处：《中华微生物学和免疫学杂志》2012年第6期486-490,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（NSFC）（20972039）;河北省自然科学基金石药集团医药联合研究基金（B2011201169）

摘　　要：目的研究含噻唑烷4．酮的糖类衍生小分子免疫调节剂对人外周血淋巴细胞活化与分化的影响。方法从健康成年人分离外周血单个核淋巴细胞，经ConA＋免疫调节剂CHla、CH2a、CHlbCH2b和匹多莫德（pidotimod）刺激48h后，收集培养上清，ELISA法检测IL-2、IL-4和IFN-y含量；72h后M1Tr法检测细胞增殖率；经ConA＋免疫调节剂CHla、CH2a、CHlbCH2b和pidotimod刺激，孵育72h后收获细胞，利用流式细胞技术检测细胞表面黏附分子。结果所有样品均可促进T细胞增殖；免疫调节剂CHla、CH2a和pidotimod上调CD3、CD4、CDl9和CDl6CD56的表达并促进IL-2和IFN-1分泌。免疫调节剂CHlb、CH2b上调CD3、CD4、CDl9和CDl6CD56的表达并促进IL-2和IL-4分泌。结论免疫调节剂CHla、CH2a和pidotimod有可能诱导Th0向Thl分化并可提高B细胞和NK细胞增殖活化水平。而CHlb、CH2b则有可能诱导Th0向Th2分化并提高B细胞和NK细胞的增殖活化水平。Objective To explore the effects of C-pseudonucleosides bearing thiazolidin-4-one as immunostimulants on differentiation and activation of human lymphocytes. Methods Peripheral blood mononuclear cells （PBMC） were isolated from healthy adults, and then incubated with immunostimulants （CH1 a, CH2a, CHlb, CH2b and pidotimod）. After 48 h, we collected the supernatants and then detected the concentrations of IL-2, IL-4 and IFN-~/ using ELISA. After 72 h, the proliferation was detected using MTI method. PBMC incubated with immunostimulants （CHla, CH2a, CHlb, CI-I2b and pidotimod）, after 72 h, the cultural cells were collected and CD expressions of lymphocytes were analyzed by flow cytometry. Results All samples could stimulate proliferation of T cells. Immunostimulants CH1 a, CH2a and pidotimod could elevate the expressions of CD3, CIM, CD19 and CD16CD56, and stimulate the secretions of IL-2 and IFN-~. Immunostimulants CHlb and CH2b could elevate the expressions of CD3, CIM, CD19 and CD16CD56, and stimulate the secretions of IL-2 and IL-4. Conclusion Immunostimulants CHla and CH2a could differentiate Th0 into Thl and promote the proliferation of B cells as well as NK cells. However, immunostimulants CHlb and CH2b could differentiate Th0 into Th2 and promote the proliferation of B cells and NK cells.

关 键 词：细胞因子 T细胞 B细胞 NK细胞 免疫调节剂 

分 类 号：R392.1[医药卫生—免疫学]