高效液相色谱紫外荧光双检测器测定蔬菜中的克百威  

High-performance Liquid Chromatography Determination of UV Fluorescence Dual Detection of Carbofuran in Vegetables

作　　者：张燕[1] 闫磊[1] 高文瑞[1] 戴洋洋 

机构地区：[1]天津市乳品食品监测中心,天津300381

出　　处：《食品研究与开发》2012年第8期156-160,共5页Food Research and Development

摘　　要：建立一种测定农产品中克百威含量的高效液相色谱法。样品经乙腈萃取后,提取液采用固相萃取技术分离、净化,经浓缩后,采用Symmetry C18色谱柱分离,流动相为甲醇∶水=35∶65(体积比),紫外检测220 nm,标准曲线R=0.999 9,最低检出限为0.033 mg/kg;荧光检测激发波长279 nm,发射波长307 nm,标准曲线R=0.999 8,最低检出限为1.6×10-3mg/kg。平均回收率分别为95.5%。To establish a determination carbofuran in vegetables content by high performance liquid chromatography. Samples with acetonitrile extraction, extraction using solid phase extraction technique for the separation, purification, after concentration, using the Symmetry C18 column, mobile phase of methanol： water = 35： 65（volume ratio ）. UV detection was 220 nm, standard curve R=0.999 9. The lowest detection limit was 0.033 mg/kg. In the fluorescence detection, excitation wavelength was 279 nm and emission wavelength was 307 nm. Standard curve R=0.999 8, the minimum detectable limit was 1.6×10^-3 mg/kg. The average recoveries were 95.5 %.

关 键 词：高效液相色谱 克百威 紫外检测器 荧光检测器 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.31[轻工技术与工程—食品科学与工程]