亚砷酸钠对人肺癌Spc-A1细胞端粒酶活性的影响  

Effect of sodium arsenite on telomerase activity of human lung carcinoma Spc-A1 cells

作　　者：施睿[1] 梁标[2,3] 

机构地区：[1]深圳市第二人民医院呼吸内科,广东深圳518035 [2]广东医学院附属医院呼吸内科 [3]广东医学院呼吸疾病研究所,广东湛江524001

出　　处：《现代肿瘤医学》2012年第9期1779-1783,共5页Journal of Modern Oncology

摘　　要：目的:研究亚砷酸钠(NaAsO2)对人肺癌Spc-A1细胞株端粒酶活性及其催化亚单位hTERT表达的影响,探讨其抗癌机制。方法:四甲基偶氮唑蓝法(MTT)检测亚砷酸钠对Spc-A1细胞增殖的抑制作用;端粒酶活性采用端粒末端重复序列扩增-酶联免疫吸附法(TRAP-ELISA)测定;端粒酶催化亚单位hTERT-mRNA表达采用反转录聚合酶链式反应法(RT-PCR)测定。结果:亚砷酸钠对人肺癌细胞株Spc-A1的增殖具有一定程度的抑制作用。在12h、24h和48h三个时间段均可见Spc-A1细胞端粒酶活性随着药物浓度的增加逐渐下降。并且,hTERT mRNA表达下调与端粒酶活性下降一致。结论:亚砷酸钠对Spc-A1细胞的增殖具有一定的抑制作用,下调癌细胞hTERT mRNA的表达来抑制端粒酶活性可能是其中一种机制。Objective：To investigate the posssible anticarcinogenic mechanisms by studying the effect of sodium arsenite （ NaAsO2 ） on telomerase activity and human telomerase reverse transcriptase （hTERE） gene expression of hu- man lung carcinoma Spe - A1 cells. Methods：MTT（ Methyl Thiazolyl Tetrazolium）assay was used to determine the growth inhibition by sodium arsenite in Spe - A1 cells. Telomerase activity was examined by telomeric repeat amplifi-cation protocol ELISA（ TRAP - ELISA）. Expression of hTERE was assessed by reverse transcription polymerase chain reaction （RT -PCR）. Results： NaAsO2 could inhibit the proliferation of Spc -A1 cells in some degree. Elomerase ac-tivity of Spc - A1 cells was declined gradually when the concentrations of NaAsO2 increased from 1 μg/ml to 2μg/ml to 4μg/ml during the period of 12h ,24h and 48h respectively. And the down - regulation of expression of hTERTmR- NA was consistent with the change of telomerase activity. Conclusion ： Sodium arsenite could obviously inhibit the pro- liferation of SPC - A - 1 cell. The inhibition of telomerase activity through decreasing expression of hTERT may be one of meehanisms.

关 键 词：亚砷酸钠 肺癌 端粒酶 人端粒酶逆转录酶 

分 类 号：R73-361[医药卫生—肿瘤] R734.2[医药卫生—临床医学]