喉癌及喉良性病变中HPV不同亚型的检测  

Detection of different type of human papillomavirus in benign,premalignant and malignant lesions of the larynx

作　　者：吴俊福[1] 周佳青[1] 

机构地区：[1]上海交通大学医学院附属仁济医院耳鼻咽喉头颈外科,上海200127

出　　处：《现代肿瘤医学》2012年第9期1813-1816,共4页Journal of Modern Oncology

基　　金：上海市教委基金(编号:101003.062)

摘　　要：目的:检测喉癌、癌前病变及喉良性病变HPVDNA表达的阳性率,探讨HPV感染与喉癌发的相关性。方法:运用流式荧光杂交法以及型特异性PCR方法对于46例喉癌组织、14例喉癌前病变组织及19例喉良性病变组织中HPVDNA进行检测分型。结果:运用流式荧光杂交法在79例喉病变标本中检测HPVDNA阳性率为10.13%,其中46例喉癌阳性率为6.52%;癌前病变组阳性率为35.71%;喉良性病变均为阴性。型特异性PCR方法检测出2例喉癌HPV16阳性,与流式荧光杂交法所检测到的2例相同。结论:HPV是喉乳头状瘤的一个独立的致病因素,而与喉癌的发生关系似乎不是很密切,但尚待进一步大样本研究。Objective：To investigate the role of human papillomavirus in benign,premalignant and malignant le-sions of the larynx. Methods： HPV was detected by flow fluorescent hybridization technology with Luminex multi - an-alytic profiling （xMAP） and Type-Specific PCR on 79 formalin -fixed and paraffin-embedded samples of larynx lesions（ include 46 laryngeal cancer - LC, 14 premalignant lesions of the larynx-PLL and 19 benign lesions of the larynx - BLL）. Results：HPV prevalence was 10.13% in these 79 samples by flow fluorescent hybridization technolo- gy with Luminex multi - analytic profiling,precancer lesions rose to 35.71%, ;and cancer cases were 6.52% ; in be-nign laryngeal lesions, HPV prevalence was 0% . HPV16 was detected in the same two positive samples as by flow fluorescent hybridization technology with Luminex multi - analytic profiling by type - Specific PCR. Conclusion ： On the basis of the results reported in this study , it seems that HPV may be considered a cause of laryngeal papillomas a-lone, as already widely reported in literature, but does not seem to be involved in the genesis of malignant laryngeal le-sions, but it needs further research from large sample.

关 键 词：喉鳞状细胞癌 喉癌前病变 人乳头状瘤病毒(HPV) 流式荧光杂交法 

分 类 号：R739.65[医药卫生—肿瘤]