检测细胞角蛋白在食管癌外周血和骨髓微转移中的意义  

Significance of cytokeratine detection for micrometastatic tumor cells in peripheral blood and bone marrow in patients with esophageal cancer

作　　者：姚成才[1,2] 张义[1,2] 柯胜奎[3] 章云鹏[4] 姜杰[5] 

机构地区：[1]福建中医药大学附属厦门中医院 [2]厦门华侨医院胸外科,福建厦门361009 [3]厦门大学附属中山医院,福建厦门361005 [4]厦门大学医学院,福建厦门361002 [5]厦门市卫生局,福建厦门361001

出　　处：《现代肿瘤医学》2012年第9期1863-1866,共4页Journal of Modern Oncology

摘　　要：目的:探讨食管癌患者外周血和骨髓中CK18mRNA基因表达及临床意义。方法:采用巢式RT-PCR方法检测50例M0、10例M1的食管癌患者及10例食管良性病变患者外周血及肋骨骨髓中细胞角蛋白CK18mRNA基因的表达。结果:50例M0食管癌患者外周血、骨髓中CK18mRNA阳性表达率分别为38.0%(19/50)和42.0%(21/50);10例M1的食管癌患者外周血、骨髓中CK18mRNA阳性表达率分别为90.0%(9/10)、100%(10/10);食管良性病变患者的外周血和骨髓中K18mRNA阳性表达率均为0%(0/10),CK18mRNA阳性表达在三组间差异有显著性(P<0.01)。CK18mRNA在Ⅳ期食管癌患者外周血和骨髓中阳性表达率与III期以前患者相比,有显著性差异(P<0.05)。CK18mRNA在外周血和骨髓中表达无差异(P>0.05),但呈正相关性(r=0.704,P<0.01)。结论:应用RT-PCR法检测食管癌患者外周血或骨髓中CK18mRNA微转移有助于食管癌的分期和预后的判断。Objective ：To explore the clinical significance of cytokeratine （CK） 18mRNA gene expression in pe-ripheral blood and bone marrow of patients with esophageal cancer. Methods： Nested reverse transcription polymerase chain reaction（ RT-PCR）was used to detect the expressions of CK18mRNA gene in peripheral blood and rib marrow of 50 esophageal carcinoma patients without distal metastasis, 10 esophagealcarcinoma patients with distal metastasis, 10 benign esophageal disease patients. Results ： In peripheral blood and rib marrow of 50 esophageal carcinoma pa- tients without distal metastasis group, the positive expression rates of CK18mRNA were 38.0% （19/50） and 42.0% （21/50）. In 10 esophageal carcinoma patients with distal metastasis group, the positive expression rates of CK18mRNA were 90.0% （9/10） and 100% （10/10）,CK18mRNA was not detected in benign esophageal disease group. There were very significant differences among three groups（ P 〈 0.01 ）. The CK18mRNA positive expression rate were significantly different in stage IV than less than stage Ⅲ esophagenal cancer （P 〈 0.05）. CK18m RNA pos-itive expression rate was not significantly different in peripheral blood and rib marrow of esophagenal cancer （ P 〉 0.05 ）, but both was closely positive correlated （r = 0. 704 ,P 〈 0.01 ）. Conclusion： It is helpful to detect the expres-sion of CK18mRNA gene in peripheral blood and rib marrow in early diagnosis of esophageal cancer micrometastasis.

关 键 词：食管癌 细胞角蛋白 微转移 外周血 骨髓 

分 类 号：R735.1[医药卫生—肿瘤]