Overexpression of Annexin A2 Is Associated with Abnormal Ubiquitination in Breast Cancer  

作　　者：Shishan Deng Baoqian Jing Tianyong Xing Lingmi Hou Zhengwei Yang 

机构地区：[1]Department of Anatomy,North Sichuan Medical College,Nanchong 637007,China [2]Institute of Immunology and Molecular Biology,North Sichuan Medical College,Nanehong 637007,China [3]Department of General Surgery,The Affiliated Hospital of North Sichuan Medical College,Nanchong 637007,China [4]Morphometric Research Laboratory,North Sichuan Medical College,Nanchong 637007,China

出　　处：《Genomics, Proteomics & Bioinformatics》2012年第3期153-157,共5页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by grants from the Sichuan Science and Technology Support Program (Grant No.2009SZ0116);Natural Science Foundation of China(Grant No. 81172496);Sichuan Department of Education (Grant No. 10ZA075)

摘　　要：Abnormal expression of annexin A2 contributes to metastasis and infiltration of cancer cells. To elucidate the cause of abnormal expres- sion of annexin A2, Western blotting, immunoproteomics and immunohistochemical staining were performed to analyze differentially ubiquitinated proteins between fresh breast cancer tissue and its adjacent normal breast tissue from five female volunteers. We detected an ubiquitinated protein that was up-regulated in the cancer tissue, which was further identified as annexin A2 by mass spectrometry. These results suggest that abnormal ubiquitination and/or degradation of annexin A2 may lead to presence of annexin A2 at high level, which may further promote metastasis and infiltration of the breast cancer cells.Abnormal expression of annexin A2 contributes to metastasis and infiltration of cancer cells. To elucidate the cause of abnormal expres- sion of annexin A2, Western blotting, immunoproteomics and immunohistochemical staining were performed to analyze differentially ubiquitinated proteins between fresh breast cancer tissue and its adjacent normal breast tissue from five female volunteers. We detected an ubiquitinated protein that was up-regulated in the cancer tissue, which was further identified as annexin A2 by mass spectrometry. These results suggest that abnormal ubiquitination and/or degradation of annexin A2 may lead to presence of annexin A2 at high level, which may further promote metastasis and infiltration of the breast cancer cells.

关 键 词：Annexin A2 Breast cancer IMMUNOPROTEOMICS UBIQUITINATION 

分 类 号：Q279[生物学—细胞生物学] Q513