Identification of the toxic compounds produced by Sargassum thunbergii to red tide microalgae  

作　　者：王仁君 王悠 唐学玺 

机构地区：[1]College of Life Science,Qufu Normal University [2]Marine Ecology Laboratory,Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第5期778-785,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No. 31070458);the National Key Technology R&D Program of China(No. 2010BAC68B00);the Specialized Research Program for Marine Public Welfare Industry from the State Oceanic Administration of China(Nos. 200805066,200905020,201005007);the Natural Science Foundation of Shandong Province(No.2009ZRB01461);the Encouraging Foundation for Outstanding Youth Scientists of China(No.2008BS09011);the Science and Technology Project of Institutions of Higher Education of Shandong(No.J10LC13)

摘　　要：The inhibitory effects of methanol extracts from the tissues of three macroalgal species on the growths of three marine red tide microalgae were assessed under laboratory conditions.Extracts of Sargassum thunbergii(Mertens ex Roth) Kuntz tissue had stronger inhibitory effects than those of either Sargassum pallidum(Turner) C.Agardh or Sargassum kjellmanianum Yendo on the growths of Heterosigma akashiwo(Hada) Hada,Skeletonema costatum(Grev.) Grev,and Prorocentrum micans Ehrenberg.Methanol extracts of S.thunbergii were further divided into petroleum ether,ethyl acetate,butanol,and distilled water phases by liquid-liquid fractionation.The petroleum ether and ethyl acetate fractions had strong algicidal effects on the microalgae.Gas chromatography-mass spectrometry analyses of these two phases identified nine fatty acids,most of which were unsaturated fatty acids.In addition,pure compounds of four of the nine unsaturated fatty acids had effective concentrations below 5 mg/L.Therefore,unsaturated fatty acids are a component of the allelochemicals in S.thunbergii tissue.The inhibitory effects of methanol extracts from the tissues of three macroalgal species on the growths of three marine red tide microalgae were assessed under laboratory conditions. Extracts of Sargassum thunbergii （Mertens ex Roth） Kuntz tissue had stronger inhibitory effects than those of either Sargassumpallidum （Turner） C. Agardh or Sargassum kjellmanianum Yendo on the growths of Heterosigma akashiwo （Hada） Hada, Skeletonema costatum （Grev.） Grev, and Prorocentrum micans Ehrenberg. Methanol extracts ofS. thunbergii were further divided into petroleum ether, ethyl acetate, butanol, and distilled water phases by liquid-liquid fractionation. The petroleum ether and ethyl acetate fractions had strong algicidal effects on the microalgae. Gas chromatography-mass spectrometry analyses of these two phases identified nine fatty acids, most of which were unsaturated fatty acids. In addition, pure compounds of four of the nine unsaturated fatty acids had effective concentrations below 5 mg/L. Therefore, unsaturated fatty acids are a component of the allelochemicals in S. thunbergii tissue.

关 键 词：allelopathic effects Heterosigma akashiwo Skeletonema costatum Prorocentrum micans unsaturated fatty acids gas chromatography-mass spectrometry （GC-MS） 

分 类 号：X173[环境科学与工程—环境科学]