魏氏拟尾柱虫休眠包囊细胞器的电镜酶细胞化学研究  

STUDIES ON ELECTRON MICROSCOPIC ENZYMO-CYTOCHEMISTRY OF ORGANELLES OF RESTING CYST IN Paraurostyla weissei (CILIOPHORA,HYPOTRICHIDA)

作　　者：陈灵[1] 倪兵[1] 顾福康[1] 

机构地区：[1]华东师范大学生物系,上海200062

出　　处：《Zoological Research》2000年第3期199-203,共5页动物学研究（英文）

基　　金：国家自然科学基金资助项目!( 3 9670 0 90 )

摘　　要：为研究纤毛虫在休眠状态下细胞生命活动的特征及细胞器的功能 ,应用电镜酶细胞化学方法显示 ,魏氏拟尾柱虫 (Paraurostylaweissei)休眠包囊中 ,在自噬泡位置 ,酸性磷酸酶反应颗粒形成多种形态的泡状体 ,ATP酶反应颗粒聚集形成泡状体或球状体 ,在分散分布的线粒体中于内膜位置形成少数琥珀酸脱氢酶反应颗粒。由所得结果推测 ,纤毛虫休眠包囊中的自噬泡消化现象是细胞在特殊生理条件下的重要生命活动 ,是休眠细胞中物质利用和能量获得的主要途径 ;自噬泡消化中也进行着对能量的利用过程 ;分散分布的线粒体是功能细胞器 ,它们与提供细胞生命活动能量有关。The study on the organelles and their functions in resting Paraurostyla weissei was carried out with electron microscopic enzymo cytochemistry method.The results revealed:①the activity of acid phosphatase (ACPase) was confined to the autophagic vacuoles of the different stages and the enzyme reaction particles wene made up the vacuolar bodies of various shapes;②the activity of adenosine triposphatase (ATPase) also presented in the autophagic vacuoles,and the enzyme reaction particles formed the vacuolar or spherical bodies of various sizes;③the localization of succinic dehydrogenase activity was in a few of the mitochondria separately scattered in the cytoplasm and there were a small number of the enzyme reaction granules in the mitochondrial inner membrane ranged with the out membrane although no reaction had been seen at a lot of the mitochondria converged to each other.Thus it was supposed from the above results:①the digestion within autophagic vacuoles that was confirmed by the ACPase reaction might be a main life process which made use of the material and supplies the energy requirement for the resting cell in a physiologically special state;②the ATPase reaction was at the autophagic vacuole,that was,the lysoome,in which the digestive duration was bound up with the energy consumption;③The mitochondria that were mostly converged to each other might be the non functional organelles in the resting cell while a few of those separately scattered in the cytoplasm might be the functional organelles.

关 键 词：魏氏拟尾柱虫 休眠包囊 电镜酶细胞化学 细胞器 

分 类 号：Q25[生物学—细胞生物学]