粘虫咽侧体静止激素的初步分离纯化  

Preliminary isolation and purification of allatostatin from Mythimna separata

作　　者：欧阳迎春[1] 唐爽[1] 关雪辰[1] 方宇凌[1] 

机构地区：[1]中国科学院动物研究所

出　　处：《昆虫学报》2000年第3期285-290,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金重点项目!(396 30 0 5 0 )

摘　　要：用放射化学的方法 ,检测粘虫Mythimnaseparata幼虫脑提取物中咽侧体静止激素 (Allato statin ,AS)样的活性物质。发现粘虫幼虫脑中含有AS样的活性物质 ,可抑制离体咽侧体 (Cor poraallata,CA)的保幼激素 (Juvenilehormone ,JH)的生物合成。用 1个脑当量的幼虫脑提取物 ,对CA的JH合成的抑制率达 51%。幼虫脑提取物经胰蛋白酶水解后 ,AS活性显著降低 ,表明幼虫脑中的活性物质是肽类或蛋白质类物质。幼虫脑提取物用Sep Pak柱初步纯化 ,有活性的组分经高压液相色谱 (HPLC)分离 ,洗脱组分的AS活性测定表明 ,有AS活性的组分主要集中在组分 1～ 2 0和组分 30～ 6 0 ,其中对离体CA的JH合成的抑制大于 50 %的组分有 3、 5、 11、 4 0、 54和 6 0。In this report, a preliminary isolation of AS from the brains of larvae, Mythimna separata, is described. The AS activity material which can inhibit JH synthesis by CA occurs in the brains of last instar. The ability of brain extract to inhibit JH synthesis in vitro was tested by radiochemical assay. Using 1 brain equivalent, 51% inhibition of JH synthesis was achieved. If the brain extract was pretreated with trypsin, the inhibitory effect of the extract was greatly reduced. Assays of fractions from the reversal phase C 18 column of HPLC separation showed that materials with AS activity were eluted between 5 2% to 28% methanol (fraction 1～20), and between 40% to 76% methanol (fraction 30～60). Assays of fractions 3, 5, 11, 40, 54 and 60 using 5 brain equivalents per pair of CA showed >50% inhibition of JH synthesis. Further purification of the fractions from the C 18 column with allatostatic activity is in progress.

关 键 词：粘虫 咽侧体 静止激素 保幼激素 分离 纯化 

分 类 号：Q965[生物学—昆虫学]