黑曲霉蛋白酶缺陷突变体的分离和鉴定  

ISOLATION AND CHARACTERIZATION OF MUTANTS OF ASPERGILLUS NIGER DEFICIENT IN EXTRACELLULAR PROTEASES

作　　者：洪斌[1] 张洋[1] 李元[1] 

机构地区：[1]中国医学科学院

出　　处：《微生物学报》2000年第4期400-405,共6页Acta Microbiologica Sinica

基　　金：国家高技术计划生物技术领域青年科学基金!资助项目 ( 863 ) ( 2 2 7)

摘　　要：对黑曲霉 (Aspergillusniger) 3.795进行紫外诱变和筛选 ,获得了胞外蛋白酶缺失的菌株。其中突变株 3 795 1、3 795 1 2 3和 3 795 1 30的生长特性与原株基本相同 ,胞外蛋白酶的活性明显低于原株 ,葡萄糖淀粉酶的活性与原株基本相同。突变株 3 795 1 2 3和3 795 1 30的胞外蛋白酶活性分别为原株的 5 4%和 8 4% ,可作为外源基因高效分泌表达的宿主菌。Several mutants of Aspergillus niger 3 795 , deficient in extracellular protease expression, have been isolated and characterized biochemically after in vivro UV mutagenesis of conidiospores and selected by halo screening on the gelatin/casein plate and skim milk plate. Extracellular proteolytic activities of 3 795 1 23 and 3 795 1 30 are 5.4% and 8.4% of the parental strain and these strains may be used as the host for efficient expression of foreign proteins.

关 键 词：黑曲霉 胞外蛋白酶 缺失菌株 外源蛋白隆解 

分 类 号：Q933[生物学—微生物学] Q78