Aβ25～35海马注射对大鼠海马硫化氢/胱硫醚β-合酶体系的影响  

Effect of Aβ25 ～ 35 microinjection on hydrogen sulfide / cystathionine-β-synthase system in the hippocampus of rats

作　　者：陈涛[1] 贾佳[2] 周发明[1] 周少华[1] 

机构地区：[1]湖北医药学院附属人民医院神经内科,442000 [2]遵义医学院附属医院消化内科,563000

出　　处：《中国老年保健医学》2012年第4期79-81,共3页Chinese Journal of Geriatric Care

基　　金：湖北教育厅资助项目;编号No.B20112015

摘　　要：目的观察Aβ25～35海马注射对大鼠海马硫化氢(hydrogensulfide,H2S)/胱硫醚β-合酶(cystathionine-β-syn-thase,CBS)体系产生的影响。方法通过双侧海马内注射Aβ25～35制作大鼠AD模型,对照组双侧海马内注射生理盐水。采用全自动酶标仪测定各组大鼠海马中H2S含量、CBS活性。结果 AD组与生理盐水组比较,海马H2S含量均明显降低(P<0.01),CBS活性显著增高(P<0.05)。结论 Aβ25～35海马注射可导致大鼠海马H2S水平下降,CBS表达增强,内源性H2S与CBS之间可能存在负反馈调节作用。Objective To investigate the effect of microinjection of Aβ25 ～35 on hydrogen sulfide （ H2S） /cystathionine-β-syn-thase （ CBS） system in the hippocampus of rats. Methods A model of AD was induced by microinjection of Aβ25 ～ 35 into thehippocampus in SD rats. And the NS group induced by microinjection of normal saline into the hippocampus. The H2S levels,andCBS activities in hippocampus in three groups were determined using a Universal Microplate Reader. Results Compared with NSgroup,H2S content in hippocampus was significantly decreased （ P 0. 01） while the expression of CBS activities was up-regulated（ P 0. 05） in AD group. Conclusion microinjection of Aβ25 ～35 into the SD rats hippocampus can decrease H2 S level and up-regulate the expression of CBS activity in thehippocampus of rats. There maybe negative feedback effect between H2 S and CBS in ratshippocampus.

关 键 词：阿尔茨海默病 Aβ25~35硫化氢 胱硫醚Β-合酶 海马 

分 类 号：R749.16[医药卫生—神经病学与精神病学]