大鼠粪样中几种糖类物质的结构确定  

Identification and Structural Determination of Saccharides in Rat Feces

作　　者：田园[1,2] 唐惠儒[1] 

机构地区：[1]波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),武汉430071 [2]中国科学院研究生院,北京100049

出　　处：《波谱学杂志》2012年第3期361-371,共11页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(20825520,21175149)

摘　　要：粪样代谢组能够反映宿主和肠道菌群相互作用的共代谢信息,然而粪样中糖类物质的归属信息迄今仍不完善.该研究使用1DNMR技术检测到大鼠粪样中木糖、阿拉伯糖、半乳糖等单糖以及由阿拉伯糖和木糖组成的寡聚糖.在使用固相萃取(SPE)方法进行分离富集的基础上,结合使用2D NMR实验技术(1 H-1 H COSY,1 H-1 H TOCSY,1 H-13 C HSQC,1 H-13 C HMBC以及DOSY)确定了这些糖类物质的结构,并对其1 H NMR和13 C NMR谱峰进行了归属.Fecal metabonomic NMR analysis has been found to offer important informa-tion for the complex interactions between mammalian metabolisms and their gut micro-biome. In this study, we set to identify and assign saccharides signal from the NMR spectra of rat fecal samples. C18 solid-phase extraction was used to extract the saccha- rides from the fecal samples. The identities of the saccharides was elucidated by atomic connectivity and diffusion coefficient data obtained from 2D NMR data including 1^H-^1H COSY, ^1H-^1H TOCSY, ^1H-^13C HSQC, ^1H-^13C HMBC and ^1H DOSY. The saceharides identified included xylose, arabinose, galactose and arabinoxylan.

关 键 词：核磁共振(NMR) 固相萃取(SPE) 2D NMR技术 粪样 低聚糖 

分 类 号：O482.53[理学—固体物理]