雷帕霉素对乳腺癌细胞株MDA-MB-231生长和凋亡的影响  

Effects of Rapamycin on Cell Growth and Apoptosis of Human Breast Cancer MDA- MB-231 cells

作　　者：马湘一[1] 刘荣华[1] 罗丹枫[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《世界最新医学信息文摘》2012年第1期1-4,共4页World Latest Medicine Information Electronic Version

基　　金：教育部新青年教师基金资助项目（200804871030）

摘　　要：背景与目的：探讨雷帕霉素（rapamycin，RAPA）在体外对乳腺癌细胞株MDA—MB-231生长和凋亡的影响及其可能的分子机制。方法：选择人乳腺癌细胞系MDA-MB-231为研究对象，经不同浓度的雷帕霉素处理后，MTT实验检测肿瘤细胞的生长抑制率，流式细胞仪检测细胞凋亡，Westem印迹法检测乳腺癌细胞中bcl-2、survivin蛋白的表达。结果：雷帕霉素可使乳腺癌细胞株MDA—MB-231增殖受到抑制并发生明显凋亡，且随时间延长作用越显著，经雷帕霉素作用后的乳腺癌细胞表达survivin、bcl-2较用药前明显下降，差异有统计学意义（P〈O．05）。结论：雷帕霉素可显著抑制乳腺癌细胞株MDA-MB-231的增殖和凋亡，其机制可能与凋亡调控基因bcl-2、survivin的表达水平变化有关。BACKGROUND＆ OBJECTIVE To investigate the effects of rapamycin on cell growth and apoptosis of breast cancer cells line MDA-MB-231, and to elucidate its possible molecular mechanism. METHODS Methyl thiazolyl tetrazolium （MTT） and flow cytometry （FCM） were used to study the effects of rapamycin induced apoptosis in breast cancer cells line MDA-MB-231. The expressions of bcl-2,and surviving were measured by Western blotting. RDSULTS The inhibitory rate and apoptosis rate of MDA-MB-231 cells treated with rapamy- cin were higher than those in the negative control group（P〈0.05）, and with concentration-dependent and time- dependent effects. The expression of survivin and bcl-2 of MDA-MB-231 cell declined obviously than that before medication. CONCLUSIONS Rapamycin in combination with paclitaxel leads to a significant apoptosis of neoplasm cells by inhibiting the expression ofbcl-2 and survivin.

关 键 词：雷帕霉素 乳腺癌 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]