Design of OFF/ON fluorescent thiol probes based on coumarin fluorophore  

作　　者：SUN Wei LI Jing LI WenHua SU LiJuan DU LuPei LI MinYong 

机构地区：[1]Key Laboratory of Chemical Biology of Natural Products(MOE),Department of Medicinal Chemistry,School of Pharmacy,Shandong University,Jinan 250012,China

出　　处：《Science China Chemistry》2012年第9期1776-1780,共5页中国科学（化学英文版）

基　　金：financed by grants from Shandong Natural Science Foundation (JQ201019);Independent Innovation Foundation of Shandong University,IIFSDU (2010JQ005)

摘　　要：This paper presents a series of first- and second-generation click-modified coumarin-based fluorescent probes for thiols. These molecules demonstrate high turn-on fluorescent response and good selectivity towards aromatic thiols over other relevant reac- tive sulfur species, reactive oxygen species and common nucleophiles. Moreover, probe la can detect thiols in the reduced rabbit plasma sample. Therefore, this approach provides a particularly impressive tool for detecting thiol in biological systems.This paper presents a series of first-and second-generation click-modified coumarin-based fluorescent probes for thiols.These molecules demonstrate high turn-on fluorescent response and good selectivity towards aromatic thiols over other relevant reactive sulfur species,reactive oxygen species and common nucleophiles.Moreover,probe 1a can detect thiols in the reduced rabbit plasma sample.Therefore,this approach provides a particularly impressive tool for detecting thiol in biological systems.

关 键 词：COUMARIN fluorescent probe aliphatic thiols THIOPHENOLS 

分 类 号：O621.2[理学—有机化学]