Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil  

作　　者：LIU Tao WANG FengHua GUO LanPing LI XiaoLiang YANG XiaoJin LIN Ai Jun 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse(Tongji University),Shanghai 200092,China [2]Experimental Teaching Center of Environmental Science and Engineering,Tongji University,Shanghai 200092,China [3]Department of Environmental Science and Engineering,Beijing university of Chemical Technology,Beijing 100029,China [4]Department of Soil Environmental Sciences,Research Center,for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [5]Institute of Chinese Material Medical,China Academy of Chinese Medical Seiences,Beijing 100700,China [6]Chinese Academy for Environmental Planning,Beijing 100012,China

出　　处：《Science China Chemistry》2012年第9期1968-1975,共8页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (40901149,81072989);the Foundation of the State Key Laboratory of Pollution Control and Resource Reuse Tongji University),China;the Chinese Universities Scientific Fund (ZD0904)

摘　　要：Two hydrocarbon-biodegrading bacterial strains, B1 and B2, were isolated from petroleum-contaminated soil collected from Tianjin, China. The strains were identified as Pseudomonas aeruginosa （B 1） and Acinetobacter junii （B2）. The degradation rate of n-hexadecane by BI and B2 reached 96% and 78% respectively after 7 days, though the strains employed different mechanisms of degradation. The results showed that B2 was not able to use glucose as carbon source. B 1 could produce glyco- lipid surfactants using glucose as the carbon source, according to the results of blue agar plate analysis and thin layer chroma- tography （TLC）, and the bacterial culture of B 1 had a high oil discharge and emulsification activity. Both B I and B2 could produce biosurfactants with hexadecane as the sole carbon source, but their modes of action were different. The carbon source was found to affect the cell surface hydrophobicity. Cell surface hydrophobicity was poor with glucose as the carbon source, but enhanced when hexadecane was used as the carbon source.Two hydrocarbon-biodegrading bacterial strains,B1 and B2,were isolated from petroleum-contaminated soil collected from Tianjin,China.The strains were identified as Pseudomonas aeruginosa(B1) and Acinetobacter junii(B2).The degradation rate of n-hexadecane by B1 and B2 reached 96% and 78% respectively after 7 days,though the strains employed different mechanisms of degradation.The results showed that B2 was not able to use glucose as carbon source.B1 could produce glycolipid surfactants using glucose as the carbon source,according to the results of blue agar plate analysis and thin layer chromatography(TLC),and the bacterial culture of B1 had a high oil discharge and emulsification activity.Both B1 and B2 could produce biosurfactants with hexadecane as the sole carbon source,but their modes of action were different.The carbon source was found to affect the cell surface hydrophobicity.Cell surface hydrophobicity was poor with glucose as the carbon source,but enhanced when hexadecane was used as the carbon source.

关 键 词：HEXADECANE BIOSURFACTANT HYDROPHOBICITY EMULSIFICATION bacteria 

分 类 号：TQ921.1[轻工技术与工程—发酵工程] X53[环境科学与工程—环境工程]