低聚木糖对大豆生长和花叶病毒病抗性的影响  

Effect of Xylo-oligosaccharide on Growth and Resistance to SMV in Soybean

作　　者：张秀秀[1] 伍辉军[1] 周宇骋[1] 高学文[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095

出　　处：《大豆科学》2012年第4期621-624,629,共5页Soybean Science

基　　金：国家转基因生物新品种培育重大专项(2011ZX08004-004);国家自然科学基金(31100056);高等学校博士学科点专项科研基金(20100097120011)

摘　　要：用不同浓度的低聚木糖处理大豆,研究低聚木糖对大豆生长和花叶病毒病(SMV)抗性的影响,温室实验结果表明:低聚木糖溶液处理大豆种子18 d后与对照相比,50 mg.L-1低聚木糖对大豆幼苗的株高、鲜重和主根长有明显的促进作用。低聚木糖喷雾处理大豆叶片18 h后接种SMV,与对照相比显著降低了SMV病情指数。利用Quantita-tive RT-PCR检测低聚木糖处理的大豆叶片中的防卫相关基因的表达情况,与对照相比,防卫基因PR2、PR10、PR12和LOX2在低聚木糖处理12 h时有最高的相对表达量;PR3在低聚木糖处理18 h时有最高的相对表达量;PR1、PAL、PPO和CHS在低聚木糖处理24 h时显著上调表达。研究表明,低聚木糖激活了大豆SA信号通路和JA信号通路,从而提高了大豆对SMV的抗性。Treated soybean with different concentrations of xylo-oligosaccharide to study the effect of xylo-oligosaccharide on growth and resistance to soybean mosaic virus（SMV）in soybean.The results of greenhouse experiment showed that 18 days after the seed of soybean were dealt with the xylo-oligosaccharide solution,compared with the contol,50 mg·L-1 xylo-oligosaccharide obviously increased the height,fresh weight,and main root length of soybean seedlings.Inoculated with SMV after 18 hours of pre-atomized the soybean leaves with xylo-oligosaccharide,compared with the contol,the disease index of soybean mosaic virus was obviously reduced.Using Quantitative RT-PCR to test the expression of the defense-related gene of the soybean leaves dealt with xylo-oligosaccharide,compared with the contol,the defense-related gene PR2,PR10,PR12 and LOX2 had the highest related expression quantity at the time of 12 hours,PR3 at 18 hours,but PR1,PAL,PPO,and CHS at 24 hours.Results suggest that xylo-oligosaccharide can active salicylic acid（SA）and jasmonic acid（JA）signaling pathways of soybean,and enhances its resistance against SMV.

关 键 词：低聚木糖 大豆 促生 SMV抗性 Quantitative RT-PCR 

分 类 号：S565.1[农业科学—作物学]