nHA/BG涂层与Bio-Oss骨粉修复种植体骨缺损的实验研究  

Experimental study of osseointegration of nHA/BG coating and Bio-Oss in implant defects

作　　者：孙菲[1] 林婷婷[1] 袁晓烨[1] 曾绍先 杜瑞林 陆尔奕[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔医学院口腔修复科上海市口腔医学重点实验室,上海200011 [2]上海海赛科技发展有限公司,上海200333

出　　处：《上海口腔医学》2012年第4期378-383,共6页Shanghai Journal of Stomatology

基　　金：上海市科学技术委员会资助项目(0852NM07200);上海市重点学科建设项目(S30206);上海市科学技术委员会实验动物项目(11140902002)~~

摘　　要：目的:评价生物玻璃/纳米羟基磷灰石(nHA/BG)涂层与Bio-Oss骨粉在种植体骨缺损中引导骨再生的效果。方法:选取6只Beagle犬,拔除两侧下颌前磨牙。3个月后预备种植窝,同时颊侧制造裂隙状骨缺损(2.25 mm×3 mm×4 mm)。按照分组植入种植体和骨粉,A组为nHA/BG+Bio-Oss,B组为nHA/BG+血凝块愈合,C组为微米级HA+Bio-Oss。术后2周、处死前2周和3 d分别进行四环素、钙黄绿素和茜素红荧光标记。术后8周和16周处死动物,行大体观察和组织学测量。采用SPSS13.0软件包对数据进行统计学分析。结果:3组骨-种植体结合率(BIC)在8周时分别为30%、18%、21%,16周时分别为61%、53%、46%;缺损区新骨面积(RA)在8周时分别为(2.1±0.6)mm3、(1.4±1.0)mm3、(0.6±0.1)mm3,16周时分别为(4.2±0.7)mm3、(2.2±1.2)mm3、(1.2±0.6)mm3。各组8周与16周的BIC和RA相比均有显著差异(P<0.05);8周时,A、C 2组的BIC和RA相比有显著差异(P<0.05),16周时,A、B 2组的RA相比有显著差异(P<0.05)。结论:在种植体周围2.25 mm骨缺损区,nHA/BG涂层能促进种植体-Bio-Oss替代骨-骨的骨结合。PURPOSE： To evaluate bone regeneration in defects at titanium implants with nHA/BG coating in conjunction with Bio-Oss.METHODS： Four mandibular premolars were extracted in 6 Beagle dogs.After 3 months,buccal dehiscence-type defects（2.25 mm×3 mm×4 mm） were surgically created following implant site.The three treatment modalities were randomly allocated： nHA/BG implant/Bio-Oss,nHA/BG implant/blood clot,and mHA implant/Bio-Oss.After 8 and 16 weeks,the dogs were sacrificed respectively.A histomorphometric analysis was performed.The data was analyzed with SPSS 13.0 software package for Student’s t test and ANOVA.RESULTS：At 8-and 16-week,nHA/BG implant group revealed comparable mean BIC（30% to 18% versus 61% to 53%）.However,mHA implant/Bio-Oss group revealed significantly lower mean BIC（21% versus 46%） values than nHA/BG implant/Bio-Oss group.A significant difference was observed for the mean BIC and RA values at 8-week between nHA/BG implant/Bio-Oss group and mHA implant/Bio-Oss group.CONCLUSIONS： It is concluded that the application of Bio-Oss graft did not seem to interfere with the nHA/BG coating activity,but ensured a stabilization of the newly formed bone for defects.

关 键 词：生物玻璃/纳米羟基磷灰石涂层 BIO-OSS骨粉 种植体 骨缺损 骨结合 

分 类 号：R783.6[医药卫生—口腔医学]