活性钙离子液对离体牙超微形态的影响与细胞毒性评价  

The influence of active calcium ions solution on the ultrastructure of the enamel and cementum of extracted teeth and the evaluation of its cytotoxicity

作　　者：毕玮[1] 余优成[1] 龚逸明[1] 周康[2] 赵青华 胡美玉[2] 

机构地区：[1]复旦大学附属中山医院口腔科,上海200032 [2]复旦大学附属中山医院中心实验室,上海200032 [3]上海力昂生物离子钙技术应用有限公司,上海201201

出　　处：《上海口腔医学》2012年第4期402-406,共5页Shanghai Journal of Stomatology

摘　　要：目的:研究活性钙离子液对离体牙牙釉质和牙骨质超微形态的影响以及对小鼠胚胎成纤维细胞NIH3T3的细胞毒性,从而为其临床应用提供依据。方法:应用Quanta200FEG场发射扫描电镜检测钙离子液成分及含量;观察钙离子液作用于离体牙前后,牙釉质和牙骨质的超微形态;MTT比色法观察不同浓度钙离子液对小鼠胚胎成纤维细胞NIH3T3细胞生长的影响,计算细胞相对增殖率并进行细胞毒性分级。结果:钙离子液主要成分为钙、碳、氧、氯。通过扫描电镜观察,钙离子液浸泡离体牙后,牙釉质和牙骨质无变化。细胞毒性分级合格的钙离子液的最大浓度为0.344 mg/mL。结论:活性钙离子液能应用于口腔,但需选择适合的浓度。PURPOSE： To detect the effect of active calcium ions solution on the ultrastructure of enamel and cementum of the extracted teeth and observe the cytotoxicity of the solution on NIH3T3 cell through cell culture.METHODS： Active calcium ions solution composition was detected with a Quanta200FEG field emission scanning electron microscopy,and the ultrastructure of teeth enamel and cementum was observed before and after the application of calcium ions solution.After diluting the calcium ions solution,MTT assay method was used to observe the impact of active calcium ions solution of different concentrations on the NIH3T3 cells growth.Based on cell relative growth rate,the cytotoxicity grade was rated.RESULTS： Active calcium ions solution was composed of calcium,carbon,oxygen,and chlorine.And there was no change in the enamel and cementum before and after soaking the teeth with active calcium ions solution.The maximum concentration of safe calcium ions solution on cytotoxicity grade was 0.344 mg/mL.CONCLUSIONS： Active calcium ions solution can be used in the oral cavity,but appropriate concentration should be selected.

关 键 词：活性钙离子液 细胞毒性 MTT比色法 

分 类 号：R783[医药卫生—口腔医学]