αB-crystallin在口腔疣状癌中的表达及其抗凋亡机制的初步研究  

Study of αB-crystallin and its possible role of anti-apoptosis in oral verrucous carcinoma

作　　者：全宏志[1] 唐瞻贵[1] 赵丽莉[1] 姚志刚[2] 王柏盛[1] 谢尚[1] 

机构地区：[1]中南大学湘雅医院.口腔医学院口腔颌面外科,湖南长沙410008 [2]中南大学湘雅医院.口腔医学院口腔病理科,湖南长沙410008

出　　处：《上海口腔医学》2012年第4期432-436,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30872895);湖南省科技厅科技计划重点项目(2008FJ2011)~~

摘　　要：目的:探讨αB-crystallin在口腔疣状癌中的表达及其抗细胞凋亡的可能机制。方法:采用免疫组织化学技术,检测αB-crystallin、活化型caspase-3在口腔疣状癌、口腔鳞癌、正常口腔黏膜的表达,并对两者的相关性进行分析。所有数据均由SPSS16.0软件包进行处理,不同组间差别采用Mann-Whitney U、Kruskal-Wallis检验;相关性分析采用Spearman等级相关分析。结果:αB-crystallin在口腔疣状癌、口腔鳞癌的表达显著高于正常口腔黏膜(P<0.05),口腔疣状癌中αB-crystallin表达的增高伴随着活化型caspase-3表达的下降(P<0.05)。结论:αB-crystallin可能通过抑制caspase-3的活化发挥抗凋亡作用,并可能在口腔疣状癌的发生、发展中发挥一定作用。PURPOSE：To investigate the expression of αB-crystallin and its possible role of anti-apoptosis in oral verrucous carcinoma.METHODS： The expression of αB-crystallin and activated caspase-3 was detected in oral verrucous carcinoma,oral squamous carcinoma and normal mucosa by immunohistochemistry,and their relationship was investigated.SPSS 16.0 software package was used for statistical analysis.Nonparametric test and spearman correlation test were performed.RESULTS： The expression of αB-crystallin in oral verrucous carcinoma and oral squamous carcinoma was significantly higher than that in normal mucosa（P〈0.05）.And in oral verrucous carcinoma,the increase of expression of αB-crystallin coincided with the decrease of expression of activated caspase-3（P〈0.05）.CONCLUSION： αB-crystallin may play a role of anti-apoptosis by inhibiting the activation of caspase-3 in oral verrucous carcinoma.

关 键 词：口腔疣状癌 αB-crystallin Activatedcaspase-3 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]