Podoplanin在口腔鳞状细胞癌中的表达及与淋巴结转移的关系  

Study on relationship between expression of podoplanin and lymph node metastasis in oral squamous cell carcinoma

作　　者：任文豪[1] 高岭[1] 徐燕[1] 赵璐[1] 郅克谦[1] 齐红[2] 荔鹏[1] 

机构地区：[1]西安交通大学医学院附属口腔医院口腔颌面外科/头颈肿瘤外科,陕西西安710004 [2]西安交通大学医学院附属口腔医院病理科,陕西西安710004

出　　处：《上海口腔医学》2012年第4期437-441,共5页Shanghai Journal of Stomatology

基　　金：陕西省卫生厅科学研究基金(2010D37);陕西省科技攻关项目(2009K17-03;2010K14-03);西安交通大学中央高校基本科研业务费专项资金(2010119)~~

摘　　要：目的:采用淋巴管特异性标志物podoplanin标记口腔鳞癌组织和正常口腔组织中的淋巴管,并计数淋巴管密度(lymphatic vessel density,LVD)值,探讨口腔鳞癌及癌周淋巴管密度与淋巴结转移的相关性及可能机制。方法:采用免疫组织化学方法检测21例正常口腔黏膜组织和88例口腔鳞癌患者组织的podoplanin表达;用podoplanin标记淋巴管,并计数口腔鳞癌和癌周组织的淋巴管密度。采用SPSS13.0软件包对数据进行t检验,分析正常组织和癌组织、淋巴结转移组和未转移组的淋巴管密度(癌周、癌内)。结果:口腔鳞癌及癌周组织的淋巴管密度均高于正常口腔黏膜组织,有显著差异(P<0.05)。癌组织内淋巴管小而闭锁,癌组织周围的淋巴管大而扩张;淋巴结转移组的癌周淋巴管密度(14.270±4.610)显著高于无转移组(9.450±2.411),差异具有显著性(P<0.05),癌组织淋巴管密度在2组间无显著差异。结论:口腔鳞癌患者癌周淋巴管的生成可能是影响区域淋巴结转移的重要因素。PURPOSE： In oral squamous cell carcinoma（OSCC） and normal oral tissues,podoplanin was used to mark lymphatic tract and recorded the lymphatic vessel density（LVD） by corresponding tissue.The aim of this study was to evaluate LVD in correlation with lymphnode metastases in patients with OSCC.METHODS：The expressions of VEGF-C antibodies and anti-podoplanin were detected in 21 normal oral tissues and 88 paraffin sections of OSCC by immunohistochemistry（S-P）;Determination of LVD（assessed by immunostaining for podoplanin） was performed as suggested by Weidner,the numerical value expressed by x±s.Intratumoral lymphatic vessels（I-LVD）and peritumoral lymphatic vessels（P-LVD） were assessed,respectively.Data was analyzed with SPSS 13.0 software package and t test was used for comparative analysis of LVD between normal oral tissues and OSCC tissue and LVD（including P-LVD and ILVD） of the lymph node metastasis and without lymph node metastasis.RESULTS： There was significant difference between LVD of normal control tissues,peritumoral and intratumoral tissues.The intratumoral lymphatic vessels（ILVD） were small and collapsed,while the peritumoral ones were large and dilated.The PLVD’s density with lymph node metastasis（14.270 ±4.610） was significantly higher than that without lymph node metastasis（9.450 ±2.411,P 〈0.05）.CONCLUSION： The results indicated that peritumoral lymphangiogenesis may have an important impact on lymph node metastasis in OSCC.

关 键 词：口腔鳞状细胞癌 PODOPLANIN 癌周淋巴管密度 癌组织淋巴管密度 淋巴管生成 

分 类 号：R739.8[医药卫生—肿瘤]