贵州省幽门螺杆菌临床菌株的抗生素耐药现状  

Antibiotic resistance of Helicobacter pylori in Guizhou Province

作　　者：刘苓[1] 胡林[1] 刘娅琳[1] 周力[1] 车筑萍[2] 谭庆华[2] 

机构地区：[1]贵州省贵阳医学院附属医院消化内科,贵州贵阳550004 [2]贵州省贵阳医学院附属医院内镜中心,贵州贵阳550004

出　　处：《胃肠病学和肝病学杂志》2012年第8期702-705,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：贵州省优秀科技教育人才省长专项资金项目;黔省专合字(2008)94号

摘　　要：目的检测贵州省幽门螺杆菌(Helicobacter pylori,H.pylori)临床菌株对多种抗生素的耐药率,并分析甲硝唑(MTZ)、克拉霉素(CLA)、阿莫西林(AMO)耐药菌株的临床特征。方法以贵州地区30株培养成功的H.pylori临床菌株为研究对象,采用Kirby-Bauer及E-test法测定菌株对MTZ、CLA、AMO等多种抗生素的耐药性。分析MTZ、CLA、AMO耐药菌株与敏感菌株患者性别、年龄及疾病种类的差异。结果 Kirby-Bauer法显示H.pylori临床菌株对AMO、左氧氟沙星、庆大霉素、CLA、四环素、头孢呋肟、复方新诺明的耐药率分别为20.00%、3.33%、13.33%、30.00%、6.67%、0、0;E-test法显示H.pylori临床菌株对MTZ、AMO、CLA耐药率分别为63.33%、26.67%、33.33%;两种检查方法检测结果无明显差异;男性患者MTZ的耐药率(77.78%)较女性患者(41.67%)高(P<0.05);30～60岁年龄组患者MTZ的耐药率更高(P<0.05);CLA和AMO的耐药率与疾病种类、性别、年龄无明显关系(P>0.05)。结论贵州地区H.pylori临床菌株的耐药现状不容忽视,以男性消化性溃疡患者年龄在30～60岁对MTZ的耐药率较高。Objective To determine the Helicobacter pylori （H. pylori） resistance to multiple antibiotics and the clinical features of antibiotic-resistance patients. Methods 30 H. pylori strains were successful]y isolated. Resistance to different antibiotics, including clarithromycin （CLA） , amoxicillin （AMO） and metronidazole （MTZ） wet＇e determined by Kirby-Bauer and E-test methods. Information of clinical features was gathered. Results Kirby-Bauer results showed 20.00% , 3.33% , 13.33% , 30.00% , 6.67% , 0, 0 of the strains were resistant to AM（）, levofloxcin, gentamicin, CLA, tetracycline, cefuroxime or SMZ-TMP respectively. E-test results manifested that 63.33% , 26.67% , 33.33% of the strains were resistant to MTZ, AMO or CLA respectively. The resistanee rate of H. pylori to MTZ in male patients tients aged 30 -60 years old had a higher resistance rate to MTZ than other groups （P 〉 0.05 ）. There was no signifieant difference in CLA and AMO resistance rate among sex ages or disease. Conclusion Resistance of H. pylori t~ antibod- ies should be pay more concern in Guizhou province. Resistant rate of H. pylori to MTZ is the highest eompared with oth- er antibodies, especially in male patients with peptic ulcer around 30 -60 ages.

关 键 词：幽门螺杆菌 抗生素耐药 

分 类 号：R57[医药卫生—消化系统]