hCLP46基因在不同侵袭转移潜能乳腺癌细胞表达中的差异及其意义  

Differential expression and significance of hCLP46 in breast cancer cell line with different invasive and metastatic potentials

作　　者：姜永冬[1] 陈艳波[1] 何川[1] 潘薇[1] 庞达[1] 张国强[1] 

机构地区：[1]哈尔滨医科大学附属第三医院乳腺外科一病区,哈尔滨150081

出　　处：《实用肿瘤学杂志》2012年第4期336-339,共4页Practical Oncology Journal

摘　　要：目的探讨hCLP46基因在MCF-7和MDA-MB-231乳腺癌细胞中表达的差异性及其意义。方法采用RT-PCR的方法检测2个细胞系（乳腺癌低度侵袭转移细胞系MCF-7、中度侵袭转移细胞系MDA-MB-231）中hCLP46mRNA的表达差异。取MCF-7和MDA-MB-231细胞株进行培养，分别加入100μmol／L的转化生长因子-B（TGF-β）并设对照组，培养72h，用Western-blot方法分析P15蛋白表达。结果RT-PCR检测hCLP46结果显示，在MCF-7和MDA-MB-231细胞系中，内参基因GAPDH的表达量相近，hCLP46基因在两个细胞系中均表达，其中MDA-MB-231细胞系中的表达高于MCF-7细胞系。两个细胞系分别加入TGF-β培养72h后，与相应的对照细胞系相比，P15的表达均有降低，hCLP46基因高表达的MDA-MB-231细胞中P15表达较MCF-7细胞显著降低。结论hCLP46基因过表达可能抑制TGF-β对MDA-MB-231和MCF-7乳腺癌细胞P15基因的上调，hCLP46可能在乳腺癌的发病中起到-定作用。Objective To investigate hCLP46 expression and significance in two breast cancer cell lines with different invasive and metastatic potentials. Methods hCLP46 expression at mRNA level was detected by RT- PCR in two different breast cancer cell lines （MCF- 7 which was low metastatic and MDA -MB -231 which was medium metastatic）. MCF -7 and MDA -MB -231 cell lines were cultured with 100 μmol/L TGF - 13 for 72 hours respectively as the experimental group. MCF - 7 and MDA - MB - 231 cell lines were cultured without TGF - β for 72 hours as the control group. Western - blotting method was used to test the P15 expression in the two cell lines. Results RT - PCR results showed that the reference gene GAPDH expressed similarly in the two cell lines,and hCLP46 expression showed higher in MDA -MB -231 cell line than that in MCF -7 cell line. The expression of P15 in both two cell tines decreased after adding TGF - β ,compared with the correspond- ing control cell lines. The expression of P15 in MDA - MB -231 cell line was lower than that in MCF -7 cell line. Conclusion It was shown that over expression of hCLP46 may inhibit the expression of P15 inducted by TGF - β in MDA - MB - 231 and MCF - 7 cell line, hCLP46 maybe play a role in the development of breast cancer.

关 键 词：乳腺肿瘤 hCLP46 P15 逆转录聚合酶链反应 

分 类 号：R737.9[医药卫生—肿瘤] R34[医药卫生—临床医学]