Cd胁迫下不同耐性豆科植物根内活性氧产生与基因组DNA多态性变化  

Reactive oxygen production and genome DNA polymorphism change in root tip tissues of two leguminous species with different tolerance under Cd stress

作　　者：张芬琴[1] 张红晓[2] 沈振国[3] 

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]河南科技大学农学院,河南洛阳471003 [3]南京农业大学生命科学学院,南京210095

出　　处：《生态学杂志》2012年第9期2330-2336,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30571107和31160053)资助

摘　　要：采用溶液培养法,研究了镉胁迫对绿豆和箭舌豌豆幼苗根基因组DNA多态性和活性氧产生的影响。结果表明:Cd胁迫降低了2种植物根尖基因组DNA模板的稳定性,导致DNA多态性发生了变化,包括RAPD谱带的增加、缺失及其荧光强度的改变;同时,Cd胁迫抑制了根的伸长生长,促进了根内超氧阴离子(O2.-)产生和过氧化氢(H2O2)积累;Cd胁迫下2种植物根的伸长与其根内O2.-产生、H2O2积累以及DNA多态性呈负相关,DNA多态性与Cd胁迫下H2O2积累呈正相关。The effects of cadmium （Cd） on the reactive oxygen productions and genome DNA polymorphism changes in roots of Phaseolus aureus Roxb. cv. VC3762 （Cdsensitive species） and Vicia sativa L. cv. 333/A （Cdresistant species） seedlings were investigated using hydroponic solution culture. The results showed that Cd stress decreased the genomic template stability and induced the changes of the genome DNA polymorphism in the root tips of the two species, manifesting the variation of band intensity, loss of normal bands, and appearance of new bands. Meanwhile, Cd stress inhibited the root growth and increased the production of O2-· and the accumulation of H2O2 in the roots. The root length of the two species under Cd stress was negatively correlated with the production of O2-·, accumulation of H2O2, and genome DNA polymorphism in root tip tissues, and the genome DNA polymorphism was positively correlated with the accumulation of H2O2 under Cd stress.

关 键 词：CD胁迫 豆科植物 活性氧 DNA多态性 

分 类 号：X173[环境科学与工程—环境科学]