硫胺素通过提高线粒体氧化状态促进植物快速响应外界胁迫的研究  

Thiamin Promotes Plant Rapid Response to External Stress through Improving Mitochondrial Oxidation Status

作　　者：周俊[1] 孙爱珍[1] 曾礼漳[1] 李哲[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所、暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2012年第4期340-345,共6页Acta Laser Biology Sinica

基　　金：教育部"长江学者与创新团队计划"创新团队项目(IRT0829);国家自然科学基金--广东联合基金重点项目(U0931005);国家高技术研究发展计划(863计划)课题(2007AA10Z204)

摘　　要：硫胺素在植物应对环境胁迫刺激及植物免疫方面发挥的作用越来越受到人们的关注。本文主要研究了硫胺素对线粒体氧化还原状态的调节作用。研究发现硫胺素可以提高线粒体的氧化状态,这一效应具有浓度依赖性,当硫胺素浓度高于1 mmol/L时较为明显。此外,硫胺素还可增强植物细胞线粒体丙酮酸脱氢酶(PDH)的活性。并且在应对铝、镉胁迫时,硫胺素预处理组能较快促进活性氧的迸发。线粒体氧化状态的提高对细胞应对胁迫因子刺激,较快释放活性氧从而激活下游信号具有重要意义。More attention has been paid on studying the role of thiamin in plant systemic acquired resistance and re- sponses to environmental stresses. The role of thiamin in modulation of mitochondria redox status was investigated. We found that thiamin could promote the oxidation state of mitochondria, which was dependent on concentration and is obvi- ous when thiamin levels higher than 1 mmol/L. Thiamin could also increase the PDH activity in plant cells. In addition, in response to the environmental stresses （such as aluminum, cadmium） , plants pretreated with thiamin could promote faster reactive oxygen species （ROS） generation. Under the stimulating from outside stress factors, the increased oxida- tion state of mitochondria result in faster ROS release, which mediates activating downstream signal transduction and con- tributes to plant induced resistance.

关 键 词：硫胺素 氧化还原状态 线粒体 活性氧 

分 类 号：Q945.78[生物学—植物学]