海洋胶原肽对SD大鼠自然衰老皮肤胶原合成的促进作用研究  

The oral administration of marine collagen peptides promoting collagen synthesis in the chronological aged skin of Sprague-Dawley rats

作　　者：梁江[1,2] 裴新荣[1] 王楠[1] 张召锋[1] 王军波[1] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191 [2]中国疾病预防控制中心营养与食品安全所

出　　处：《现代预防医学》2012年第17期4378-4381,共4页Modern Preventive Medicine

基　　金：国家"十一五"科技支撑项目(2006BAD27B08)

摘　　要：目的观察海洋胶原肽(MCP)长期喂养对SD大鼠自然衰老皮肤的胶原合成的影响。方法 4周龄雄性SD大鼠按体重随机分入对照组和海洋胶原肽低、中、高3个剂量干预组,分别在基础饲料中添加0%、2.25%、4.5%和9%(wt/wt)的MCP喂养24月后取其脊柱两侧皮肤组织。结果经过MCP长期干预,免疫组织化学实验结果表明皮肤组织Ⅰ型与Ⅲ型胶原蛋白的表达水平较老年对照组明显增加;MCP3个剂量组的Ⅰ型和Ⅲ型前胶原基因COL1A2和COL3A1的表达水平均比老年对照组有显著升高(P﹤0.05);与老年对照组相比,MCP干预组TβRⅡ蛋白的表达水平及磷酸化Smad2/Smad2的比例显著升高,而Smad7的表达水平则有一定程度的下降(P﹤0.05)。结论本研究表明MCP长期干预对于SD大鼠自然衰老皮肤的胶原合成具有一定的促进作用,其机制可能与上调TβRⅡ蛋白表达水平而激活Smad蛋白信号通路而促进胶原蛋白表达有关。OBJECTIVE The aim of the study was to investigate the effect of long-term oral administration of marine collagen peptides on the collagen synthesis in the chronological aged skin.METHODS Sprague-Dawley male rats of 4-week-old were administrated with MCP at the diet concentrations of 0%,2.25%,4.5%,and 9% for 24 months.After being sacri？ced,full thickness skin samples were excised from the depilated dorsal regions.RESULTS Immuno-histological analysis showed that MCP had the potential to promote the expression of typeⅠand Ⅲ collagen in chronological aged skin.Furthermore,quantitative real-time polymerase chain reaction revealed MCP was able to promote the expressions of procollagen type Ⅰ and Ⅲ mRNA（COL1A2 and COL3A1）（P﹤0.05）.Meanwhile,as compared with the aged control group,western blot analysis indicated the expression level of TGF-βRⅡ and ratio of phosphorylated Smad2 were up-regulated in the MCP-treated groups,whereas the expression level of Smad7 markedly decreased in the MCP-treated groups（P﹤0.05）.CONCLUSION MCP is demonstrated to have the potential to promote collagen synthesis in the chronological aged skin of Sprague-Dawley male rats with the possible mechanism of activating TGF-β/Smad signaling pathway.

关 键 词：海洋胶原肽 自然衰老皮肤 胶原合成 SD大鼠 

分 类 号：Q819[生物学—生物工程]