成熟T和NK细胞淋巴瘤与EB病毒感染关系的探讨  

Investigation on the correlation between mature T and nature killer cell lymphomas with EBV infection

作　　者：周祥祯[1] 莫祥兰[1] 黄振录[1] 梁钦清[1] 

机构地区：[1]广西壮族自治区人民医院病理科,南宁530021

出　　处：《中国临床新医学》2012年第8期745-747,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

摘　　要：目的探讨成熟T和NK细胞淋巴瘤与EB病毒感染的关系。方法用原位杂交法检测121例成熟T和NK细胞淋巴瘤EBER(EB病毒编码的小RNA)表达情况,统计不同类型成熟T和NK细胞淋巴瘤与EB病毒感染相关性。结果 121例成熟T和NK细胞淋巴瘤EBER阳性率为63.6%(77/121),其中结外者EBER阳性表达率为75.9%(60/79),阳性细胞多为肿瘤细胞,阳性细胞数>30%;而结内者EBER阳性表达率为40.5%(17/42),阳性细胞多为母化的肿瘤细胞或反应性B淋巴细胞,阳性细胞数<10%。结外成熟T和NK细胞淋巴瘤EBER表达率显著高于结内(P<0.01)。EBER表达与组织学类型有关(P<0.01)。不同类型淋巴瘤EBER阳性表达率不同:结外鼻型NK/T细胞淋巴瘤(ENKCL)为98.0%(48/49),EBER阳性细胞数>30%;血管免疫母细胞性T细胞淋巴瘤(AITL)为77.8%(7/9),阳性细胞数<10%,阳性细胞多为反应性B细胞;外周T细胞淋巴瘤非特殊型(PT,NOS)为41.5%(17/41);肠病相关性T细胞淋巴瘤(EATL)为33.3%(2/6);间变性大细胞淋巴瘤并ALK阳性(ALCL,ALK+)者为18.2%(2/11)。结论结外成熟T和NK细胞淋巴瘤发生与EB病毒感染密切相关,而结内成熟T和NK细胞淋巴瘤EB病毒感染可能为继发感染。检测肿瘤组织EBER表达有助于成熟T和NK细胞淋巴瘤的诊断和治疗。Objective To investigate the correlation between mature T and nature killer（NK） cell lympho- mas with EBV infection. Methods The expression of EBER was detected using in situ hybridization method in 121 cases of mature T and nature killer cell lympbomas. Results The expression of EBER was seen in 63.6% （77/121） of the cases. The EBER positive cells were tumor cells, and more than 30% of the tumor cells were EBER expression in extranodal mature T cell and NK cell lymphomas, while in nodal mature T cell and NK cell lymphomas, the EBER positive cells were less than 10%, the positive cells were large transform tumor cells or reactive B cells. The EBER expression rate of extranodal mature T cell and NK cell lymphomas （75.9% ,60/79） was higher than that of nodal mature T cell and NK cell lymphomas （40. 5%, 17/42 ） （ P 〈 0.01 ）. The positive rate of EBER was correlated with histopathological subtypes and location of diseases （P 〈 0.01 ）. 98.0% （48/49） of extranodal NK/T cell lymphoma （ENKCL） were EBV infection and the EBER positive cells were more than 30%, 77. 8% （7/9） of angioimmunoblas- tic T cell lymphoma（AITL） were EBV infection, and the EBER expression cells were some of reactive B cells, the positive cells were less than 10% ,while most of the tumor cells were negative to EBER. 41.5% （ 17/41 ） of peripher- al T cell lymphoma non specificity （ PT, NOS ）, 33.3 % （ 2/6 ） enteropathy-associatod T-cell lymphoma （EATL） and 18.2% （2/11） of anaplastic large cell lymphoma with ALK positive ALCL（ ALK ＋ ） were EBV infection. Conclusion EBV maybe play an important role on the development of extranodal mature T and nature killer cell lymphomas. Detecting the expression profile of EBER in mature T and nature killer cell lymphomas may be helpful to their diagno- sis and therapy.

关 键 词：淋巴瘤 原位杂交法 EB病毒 

分 类 号：R733.7[医药卫生—肿瘤]