参龙健脑胶囊对脑缺血大鼠脑组织海马区BDNF和TrkB表达的影响  

Effect of Shenlong Jiannao Capsule on Expressions of BDNF and TrkB in Hippocampus of Cerebral Ischemia Rats

作　　者：王发渭[1] 许成勇[1] 黄泉智[1] 张岗[1] 陈利平[1] 

机构地区：[1]解放军总医院中医科,北京100853

出　　处：《辽宁中医药大学学报》2012年第9期14-16,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：首都市民健康项目(Z111107067311022)

摘　　要：目的:观察参龙健脑胶囊对脑缺血损伤大鼠脑组织神经元的保护作用,并探讨其作用机制。方法:用结扎双侧颈总动脉方法制备缺血性脑损伤大鼠模型,造模成功后随机分为假手术组、模型组、参龙脑胶囊低、高剂量组及脑复康组,参龙健脑胶囊低、高剂量组和脑复康组分别按照0.4、1.2、0.45g.kg-1剂量,灌胃1次/d。灌胃28天后,采用免疫组化方法观察大鼠脑组织海马区的BDNF、TrkB表达。结果:与模型组大鼠比较,参龙健脑胶囊组大鼠脑组织中海马区BDNF、TrkB阳性表达增加(P<0.01)。结论:参龙健脑胶囊对大鼠脑缺血损伤后的神经功能恢复有一定的促进作用,其机制可能与促进BDNF和TrkB表达密切相关。Objective：To observe the neurological protective effects of Shenlong Jiannao capsule on cerebral ischemia in rats and to explore its possible mechanism.Methods：Two-vessel occlusions were adopted to reproduce the cerebral ischaemia animal model.Rats were randomly divided into 5 groups as following：sham operation group,model control group,positive control group with pisacetam,Shenlong Jiannao capsule groups（low and high dosage）.Shenlong Jiannao capsule groups（low and high dosage）and pisacetam groups were treated by gavage at the dosage of 0.4,1.2,0.45g·kg-1.After 28 days,immunohistochemistry was used to assay the expressions of BDNF and TrkB in hippocampus of cerebral ischaemia rats.Result：Comparing with model group,the expressions of BDNF and TrkB were increased in Shenlong Jiannao capsule group（P0.01）.Conclusion：Shenlong Jiannao capsule could improve the neurological deficits after cerebral ischemia in rats.The mechanism may be related with increasing expressions of BDNF and TrkB.

关 键 词：参龙健脑胶囊 脑缺血 脑源性神经生长因子 酪氨酸激酶B 大鼠 

分 类 号：R22[医药卫生—中医基础理论]