盘龙参药材的质量标准研究  

Study on the Quality Standard of Spiranthes sinensis

作　　者：崔红花[1] 尹永芹[1] 王淑美[1] 彭新生[2] 赵英日[3] 罗兰[1] 陈超[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]广东医学院药学院,广东东莞523808 [3]广州中医药大学国际学院,广州510006

出　　处：《中国药房》2012年第35期3310-3312,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(20905015);广东省自然科学基金资助项目(9451022401003240);广东高校优秀青年创新人才培育项目(WYM10036);广东省科技计划项目(2011B037100058);广东省东莞市科技攻关项目(201110815200140)

摘　　要：目的:制定盘龙参药材的质量标准。方法:根据2010年版《中国药典》方法,鉴别盘龙参的性状、显微特征,采用浸出物测定法、灰分测定法、重金属测定法、砷盐测定法、水分测定法检测杂质,采用薄层色谱(TLC)法与高效液相色谱法进行定性、定量研究。结果:不同产地的盘龙参药材性状各不相同;显微鉴别常见韧皮纤维、非腺毛和导管;冷、热浸出物量分别不得低于7.0%、17.0%;总灰分不得超过7.0%,酸不溶性灰分不得超过0.4%;重金属和砷盐含量未超标;水分不得超过12.0%。TLC斑点清晰、分离度好;山柰酚进样量在0.0122～0.3050μg范围内与峰面积积分值呈良好的线性关系,平均加样回收率为99.61%,RSD=2.89%(n=6)。结论:所建标准可用于盘龙参药材的质量控制。OBJECTIVE： To establish the quality standard of Spiranthes sinensis. METHODS： According to Chinese Pharmaco- peia （2010 edition）, the property and microscopic characteristics of S. sinensis, moisture, total ashes content, acid-insoluble ash content, heavy metals, arsenic, water content were studied. Qualitative and quantitative identification were established by TLC and HPLC respectively. RESULTS： The property of S. sinensis from different producing areas was different. The microscopic identifica- tion showed there were phloem fiber, nonglandular hair and vessel. The amounts of cold and heat extracts were no lower than 7.0% and 17.0% ; total ashes content was no more than 7.0%, and acid-insoluble ash content no more than 0.4% ; the contents of heavy metals and arsenic didn＇ t exceed the standard; water content was no more than 12.0%. The spot of TLC were clear and well-separated. The linear range of kaempferol was 0.012 2 -0.305 0 μg with an average recovery of 99.61% （ RSD = 2.89 %, n = 6 ）. CONCLUSION： The established standard is suitable for quality control of S. sinensis.

关 键 词：盘龙参 质量标准 薄层色谱法 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学] R282.73[医药卫生—中医学]