带有PreS的重组乙肝表面抗原在毕赤酵母中的表达  

Expression of the Recombinant Hepatitis B Virus Surface Antigen Carrying PreS Epitopes in Pichia pastoris

作　　者：杨剑莹[1] 惠静毅[1] 李光地[1] 汪垣[1] 袁汉英[2] 李育阳[2] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031 [2]复旦大学遗传学研究所,上海200433

出　　处：《生物化学与生物物理学报》2000年第2期139-144,共6页

基　　金：国家高技术"86 3"计划资助项目 !No .10 2 0 7 0 1 0 2&&

摘　　要：带有PreS区的乙肝表面抗原 (HBsAg)有望成为新一代更高效的乙肝疫苗。利用毕赤属酵母 (Pichiapas toris)表达系统 ,表达了带有PreS区免疫决定簇的重组乙肝表面抗原S1S、SS1和S2S。对表达产物的性质鉴定表明 ,产物可以形成具有相应的S、PreS1或PreS2抗原性的颗粒 ,表达水平高于啤酒酵母 (Saccharomycescerevisiae)Many studies have suggested that hepatitis B surface antigen(HBsAg) including PreS sequences could be an ideal candidate for highly effective hepatitis B virus vaccine. Modified surface antigens S1S, SS1 and S2S which carried PreS epitopes, were expressed in Pichia pastoris. The characterization of antigenicity and particle assembly demonstrated that the expression products could be assembled into particles which presented S, PreS1 or PreS2 antigenicity, respectively. The expression was more efficient than that in Saccharomyces cerevisiae.

关 键 词：乙肝表面抗原 PRES 毕赤酵母 性质鉴定 基因表达 

分 类 号：Q786[生物学—分子生物学]