细胞周期蛋白(cyclin)D在鼻咽癌中的表达及功能初步分析  

The Primary Study on Expression and Function of D-Type Cyclins in Nasopharyngeal Carcinoma Cell Lines

作　　者：赵晓荣[1] 顾焕华[1] 翁新宪[1] 易薇[1] 邓锡云[1] 曹亚[1] 

机构地区：[1]湖南医科大学肿瘤研究所,长沙410078

出　　处：《生物化学与生物物理学报》2000年第2期192-196,共5页

基　　金：国家自然科学基金杰出青年基金 (No .3 95 2 5 0 2 2 );国家重点基础研究发展规划资助项目! ( 973 )(No .G19980 5 12 0 1)&&

摘　　要：探讨了细胞周期蛋白 (cyclin)D在EB病毒 (Epstein Barrvirus)潜伏膜蛋白 1(latentmembraneprotein ,LMP1)阳性和阴性表达的鼻咽癌细胞系中的表达特征 ,利用流式细胞仪同时从DNA及蛋白质水平初步分析了cyclinD1在鼻咽癌细胞系中的功能。Western印迹的结果发现cyclinD1在CNE LMP1中过表达 ,在HNE1中表达较弱 ;CyclinD2、cyclinD3在两株鼻咽癌细胞系中均普遍表达 ,在CNE LMP1中的表达均强于其在HNE1中的表达。流式细胞仪双参数的结果显示 ,cyclinD1在鼻咽癌细胞系中的表达呈细胞周期依赖性 ,且呈周期性振荡 ,在G1期表达最高 ,S期下降 ,但是S期及G2 /M期仍可检测到cyclinD1的表达。以上结果提示 :cyclinD1可能与G1/S期行进有关 ,cyclinD在EBV LMP1( +)的细胞系中表达增强可能与EBV LMP1的表达有关 ,为进一步探讨EBV LMP1的活化与细胞周期的紊乱提供了新的线索。Previous work of the authors established the protein expression spectra of D type cyclins, and found that there were differential expression in nasopharyngeal carcinoma (NPC) biopsies. Using Western Blotting, aberrant overexpression of the cyclin D1 protein in NPC cell lines is reported. Cyclin D2 and cyclin D3,the other members of D type cyclins, were also detected in NPC cell lines. A comparison of the expression patterns in the cell lines, positive or negative in the latent membrane protein 1 (LMP1) encoded by Epstein Barr virus, revealed that the expression of D Type cyclin proteins might be increased by EBV LMP1. The abundance of these proteins showed characteristic variation consistent with a cell cycle dependent oscillation and the peak levels expressed in G1 as analyzed by double parameter flow cytometry. These data suggest that cyclin D1 is essential for cell cycle progression in G1 and may play a role in NPC carcinogenesis.

关 键 词：细胞周期蛋白D 鼻咽癌 EB病毒 表达 

分 类 号：R739.63[医药卫生—肿瘤] R329.28[医药卫生—临床医学]