水稻基因组中MITEs的偏爱位置  

Preferential Location of MITEs in Rice Genome

作　　者：张显亮[1] 洪国藩[1] 

机构地区：[1]中国科学院国家基因研究中心,上海200233

出　　处：《生物化学与生物物理学报》2000年第3期223-228,共6页

摘　　要：已知微型倒转重复转座因子 (MITEs)广泛分布于真核生物基因附近的非编码区。为探讨MITEs在基因组一级结构中的分布规律 ,通过对 82kb的水稻BAC克隆l1 3 3 2全序列分析 ,精确鉴定了 1 0个蛋白质编码基因的位置和转录方向以及 7个MITEs的位置 ,毫无例外的观察到 ,MITEs只要存在 ,总是位于最近的基因的上游 ,不管该基因与之相距多远、转录方向如何。另外还发现 ,1个MITE被 2个基因共享。由于MITEs易于鉴定 ,因而这种位置模式可以用作探测其下游基因存在的工具。To observe the association between MITEs and genes in details, 82 kb of the rice genomic DNA were sequenced, and 10 genes were identified by similarity search. It was found that miniature inverted repeat transposable elements (MITEs) were located upstream of the genes identified without exception, regardless of their directions of transcription. One MITE was found to be located between two 5′regions of the genes with opposite orientation of transcription, indicating that it was shared by them. The positional patterns of MITEs may therefore serve as an aid for preliminary screening of genes.

关 键 词：水稻基因组 位置模式 MITES 基因5'侧区 

分 类 号：S511.101[农业科学—作物学]