SMAD4在嗜甲醇酵母中的表达及鉴定  

Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein

作　　者：刘一平[1] 杨晓[2] 徐晓玲[3] 邓继先[2] 程萱[2] 黄翠芬[2] 

机构地区：[1]广州军区医学研究所,广州510507 [2]军事医学科学院生物工程研究所,北京100071 [3]美国国立卫生研究院糖尿病,消化道病和肾病研究所,贝塞斯达md20892

出　　处：《生物化学与生物物理学报》2000年第3期239-242,共4页

摘　　要：Smad4是新近发现的一种肿瘤抑制基因。在 5 0 %的胰腺癌和 30 %的结肠癌中 ,此基因发生突变或缺失。利用Pichiapastoris酵母表达系统 ,以胞外分泌的方式表达了SMAD4蛋白 ,并对SMAD4蛋白的生化性质进行了分析。结果显示表达蛋白的分子量约 6 7kD ,经Western印迹鉴定 ,与SMAD4抗体有特异的结合反应 ,N末端Smad 4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad 4 cDNA, and it was able to specifically react with the antibody against SMAD4.

关 键 词：SMAD4 嗜甲醇酵母表达系统 鉴定 肿瘤抑制基因 

分 类 号：R730.3[医药卫生—肿瘤]