氧化修饰使HDL促动脉平滑肌细胞胆固醇流出减少  

Oxidative Modification Reduces HDL-induced Cholesterol Efflux from Cultured Human Arterial Smooth Muscle Cells

作　　者：傅强[1] 刘秉文[1] 

机构地区：[1]华西医科大学生物化学与分子生物学研究所,成都610041

出　　处：《生物化学与生物物理学报》2000年第3期248-252,共5页

基　　金：教育部博士点专项科研基金;美国纽约中华医学基金部分资助课题&&

摘　　要：为了研究氧化修饰对高密度脂蛋白 (HDL)转运细胞胆固醇的影响 ,将3 H 胆固醇负荷的培养人动脉平滑肌细胞 (SMC)分别与天然HDL及Cu2 + 或HOCl氧化修饰的HDL在 37℃温育不同时间后 ,分别测定细胞3 H 胆固醇清除率。结果发现 ,温育 2 4h后 ,经Cu2 + 或HOCl氧化修饰后的HDL其细胞胆固醇清除率分别较天然HDL下降了 30 .0 %和 43.1% (p <0 .0 1)。结果还发现 ,Cu2 + 或HOCl氧化修饰的HDL其转运细胞胆固醇的速率也比天然HDL大大降低。加入叔丁基甲苯 (BHT)、VitC及VitE可抑制Cu2 + 或HOCl诱导HDL的氧化修饰 ,使其转运细胞胆固醇的能力提高。To investigate the effects of oxidative modification of high density lipoprotein (HDL) on HDL mediated cholesterol efflux from human arterial smooth muscle cells(SMC), native HDL (N HDL) and oxidized HDL(Ox HDL) induced by copper or hypochlorite were incubated with cultured SMC preloaded with 3H cholesterol for 2—24 h. The radioactivities of 3H cholesterol of the medium and SMC were measured respectively, and the cholesterol efflux rate (CER) of HDL and Ox HDL were determined. The result showed that the CER of N HDL was 43.1% after 24 h incubation, while the CER of Ox HDL induced by copper or hypochlorite were 28.9% and 23.5% respectively, reduced by 30.0% and 43.1% compared with N HDL. The speed of 3H cholesterol efflux of Ox HDL from SMC were decreased remarkably. Inhibition of oxidation with antioxidants butylated hydroxytoluene(BHT), vitamin C or vitamin E recovered mostly the ability of HDL to stimulate cholesterol efflux. The results suggest that oxidation of HDL may attenuate its effect on cholesterol efflux from arterial SMC, and vitamin C and vitamin E supplementation may protect artery from atherogenesis.

关 键 词：HDL 氧化修饰 平滑肌细胞 RCT 抗氧化剂 AS 

分 类 号：R364[医药卫生—病理学]