滁菊总黄酮对大鼠心肌缺血再灌注损伤的影响  

Effect of Total Flavonoids from Dendranthema morifolium (Ramat) Tzvel. cv. Chuju Flowers on Myocardial Ischemia Reperfusion Injury in Rats

作　　者：俞浩[1] 肖新[1] 刘汉珍[1] 周金星[1] 马世堂[1] 张孝林[1] 

机构地区：[1]安徽科技学院食品药品学院,安徽蚌埠233100

出　　处：《食品科学》2012年第15期283-286,共4页Food Science

基　　金：安徽省自然科学基金项目(10040606Q13);科技部科技人员服务企业行动项目(2009GJC30034);安徽科技学院稳定人才项目(ZRC2011297)

摘　　要：目的:观察滁菊总黄酮对Wistar大鼠心肌缺血再灌注损伤(MIRI)的作用。方法:采用结扎冠状动脉左前降支30min后再灌注60min的方法复制急性MIRI大鼠模型,观察滁菊总黄酮对MIRI大鼠心肌梗死范围,血清肌酸激酶(CK)、乳酸脱氢酶(LDH)活性,血清及心肌中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,体外血栓长度及干、湿质量,全血及血浆黏度的影响。结果:滁菊总黄酮能显著减小MIRI大鼠心肌梗死范围,降低血清C K、L D H活性,升高血清和心肌组织中S O D活性,降低M D A含量和体外血栓长度,减轻血栓干、湿质量,改善全血黏度。结论:滁菊总黄酮对大鼠MIRI具有保护作用。Objective： To observe the effect and underlying mechanism of total flavonoids extracted from Dendranthema morifolium（Ramat） Tzvel.cv.Chuju flowers on myocardial ischemia reperfusion injury（MIRI） in rats.Methods： Acute MIRI mouse models were created by reperfusion for 60 min after anterior interventricular branch of the left coronary artery for 30 min.The infarction area of myocardium,serum creatine kinase（CK） and lactate dehydrogenase（LDH） activities,SOD activity in serum and myocardium,and MDA content in serum and heart tissue were determined.Thrombus length,dry and wet weight of thrombi,and viscosity of whole blood and plasma were also evaluated.Results： Total flavonoids rom Dendranthema morifolium（Ramat） Tzvel.cv.Chuju flowers could decrease the infarction area of myocardium,reduce serum CK and LDH activity,improve the activity of SOD in serum and heart tissue,decrease MDA content in serum and heart tissue,reduce thrombus length,wet weight and dry weight of thrombi in vitro,and improve the viscosity of whole blood.Conclusion： Total flavonoids from Dendranthema morifolium（Ramat） Tzvel.cv.Chuju flowers can protect against MIRI in rats.

关 键 词：滁菊总黄酮 WISTAR大鼠 心肌缺血再灌注损伤 脂质过氧化 

分 类 号：R965.1[医药卫生—药理学]