PARP-1激活与体外循环后全身炎症反应综合征的研究  

Study on systemic inflammatory reaction syndrome after PAPR-1 activation and extracorporeal circulation

作　　者：魏振宇[1] 李小丽[2] 孙鹏飞[1] 田行瀚[2] 梁亚凤[2] 郑磊[1] 

机构地区：[1]烟台毓璜顶医院心外科,山东264000 [2]烟台毓璜顶医院ICU

出　　处：《医学动物防制》2012年第9期961-963,共3页Journal of Medical Pest Control

基　　金：烟台市科技发展项(2009155-11)

摘　　要：目的观察体外循环后外周血单个核细胞PARP-1活性的变化规律以及PAPP-1活性和白细胞介素1β(IL-1β)、白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)的关系。方法选择烟台毓璜顶医院体外循环心脏手术病人20例为研究对象。分别于术前0min、主动脉开放后5min、术后2h、术后4h、术后24h、术后48h桡动脉抽取血标本10mL,标本离心分离血浆、单个核细胞,ELISA试剂盒检测各个时间点的IL-1β、IL-8及TNF-α的水平,PARP-1活性检测试剂盒(三氯乙酸沉淀法)检测PARP-1活性。结果体外循环后外周血单个核细胞PAPR-1活性、血浆IL-1β、IL-8和TNF-α浓度均明显升高。PAPR-1活性在术后2h达高峰后逐渐下降,TNF-α在术后2h达高峰,IL-1β与IL-8均在术后4h血浆浓度最高。结论体外循环后外周血单个核细胞PAPR-1活性明显升高,PARP-1的激活与炎症因子的升高有一定程度同步性。PAPR-1可能是体外循环后全身炎症反应综合征过程中的重要调节因子。Objective To observe the activity of peripheral blood mononuclear cell PARP-1 after extracorporeal circulation and its relationship with interleukin-1β（IL-1β）,interleukin-8（IL-8）,tumor necrosis factor-α（TNF-α）.Method Take 20 patients conducting extracorporeal circulation cardiac surgery at Yantai Yuhuangding Hospital as study object to collect 10 mL radial artery blood samples just before the surgery,5 minutes after aorta open,and 2h,4h,24h,and 48h after the surgery,respectively.Separate plasma and mononuclear cell by centrifugalization,and detect the level of IL-1β,IL-8 and TNF-α at each time point by ELISA kit,and detect the activity of PARP-1 by detection kit（method of trichloroacetonitrile precipitation）.Results The activity of peripheral blood mononuclear cell PARP-1 and the concentration of IL-1β,IL-8 and TNF-α increased significantly after extracorporeal circulation.The activity of PARP-1 reached its peak 2 hours after the surgery and then began to decrease.The concentration of TNF-α reached its peak 2 hours after the surgery and the concentration of IL-1β and IL-8 reached their pears 4 hours after the surgery.Conclusions The activity of peripheral blood mononuclear cell PARP-1 increased obviously after extracorporeal circulation,and the activation of PAPR-1 and the increase of inflammatory factor had a certain synchronism.PARP-1 might be the important regulatory factor in the systemic inflammatory reaction syndrome after extracorporeal circulation.

关 键 词：体外循环 全身炎症反应综合征 PARP1-1 细胞因子 

分 类 号：R364.5[医药卫生—病理学] R654.1[医药卫生—基础医学]