响应曲面法对蛹虫草多糖超声提取工艺的优化效果  

Optimization of Ultrasonic-assisted Extraction of Cultured Cordyceps militaris Polysaccharide by Response Surface Methodology

作　　者：邓黎[1,2,3] 周同永[1,2,3] 韩涛[1,2,3] 赵晓辉[1,2,3] 皮立[1,2,3] 韩发[1,2] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院,西北高原生物研究所,高原生物适应与进化重点实验室,青海西宁810008 [3]中国科学院研究生院,北京100049

出　　处：《贵州农业科学》2012年第8期187-190,共4页Guizhou Agricultural Sciences

基　　金：中科院科技支青项目"高活性微孔草不饱和脂肪酸提取技术及其产业化"(082B371211);国家基金项目"特种资源植物微孔草种群恢复机制及保护途径研究"(31070475)

摘　　要：为了优化蛹虫草多糖超声提取工艺,采用响应曲面法,根据中心旋转试验设计,研究超声提取时间、提取温度和液料比等工艺条件对提取率的影响。结果表明:蛹虫草多糖超声提取最佳工艺条件为超声提取时间65min、温度80℃、液固比32mL/g、提取2次,提取率可达3.89%。Response surface methodology was used to optimize polysaccharide ultrasonic-assisted extraction process from cultured C.militaris based on a central composite design（CCD）,the effects of extraction time,extraction temperature and ratio of material to liquid on the yield of polysaccharide were studied.The results showed that the optimum extraction condition was obtained as follows： ultrasonic-assisted extraction time 65 min,extraction temperature 80℃,ratio of material to liquid 32 mL/g and extraction times two,then the yield of polysaccharide was 3.889%.

关 键 词：蛹虫草 多糖 超声提取 响应曲面法 

分 类 号：S567.35[农业科学—中草药栽培] Q946.91[农业科学—作物学]