益母草水苏碱对肥大心肌细胞活性氧信号的影响  

Effect of Leonurus Stachydrine on Myocardial Cell Hypertrophy

作　　者：郭炜[1] 章忱[1] 廖月玲[1] 吕嵘[1] 卫洪昌[1] 

机构地区：[1]上海中医药大学病理教研室,上海201203

出　　处：《中药材》2012年第6期940-943,共4页Journal of Chinese Medicinal Materials

基　　金：上海市教委重点学科建设项目(J50301);上海中医药大学中青年骨干教师教学能力提升计划资助项目;上海市博士学位点建设科研项目(K110406);上海市科委重点项目(09JC1413400);上海市教委重点项目(09ZZ126)

摘　　要：目的:观察益母草水苏碱对心肌细胞肥大的作用,探讨其对活性氧(ROS)及其下游NF-κB信号的影响。方法:原代培养乳鼠的心肌细胞,用血管紧张素Ⅱ建立细胞肥大模型,观察10-7～10-4mol/L浓度的益母草水苏碱对细胞肥大的影响(测定细胞体表面积及Protein/DNA比值),同时利用流式细胞仪检测ROS阳性细胞比例,West-ern Blot法测定细胞浆内p-IκBα(ser32)及核内NF-κB(p65)蛋白表达。结果:一定浓度的益母草水苏碱可以降低肥大心肌细胞的体表面积、Protein/DNA比值以及ROS阳性细胞比例(P<0.05);益母草水苏碱干预降低了肥大心肌细胞浆内p-IκBα(ser32)及核内NF-κB(p65)蛋白的表达水平(P<0.05)。结论:对ROS及其下游NF-κB信号的抑制可能涉及益母草水苏碱对抗心肌细胞肥大的作用机制。Objective： To investigate the effects of Leonurns stachydrine on myocardial cell hypertrophy induced by angiotensin.II in rats. Methods ： Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertro- phy models. The cell surface area and protein / DNA ratio were detected as index for hypertrophy of myocardial cells. ROS positive myocardial cell were counted by means of flow cytometry, the expression of p-IκB （set32）protein in the eytosol and NF-κB （p65） protein in the nucleus were also detected by Western blot. Results： Various concentrations of staehydrine intervention （10-6-10-4mol/L） showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell （ P 〈 0.05 ）. Meanwhile, intervention of stachydrine significantly suppressed the level of p-IκB （ser32）protein in the cytosol and NF-KB （p65） protein in the nucleus （P 〈 0.05 ）. Conclusion ： Stachydrine can inhibit the NF-κB signal pathway, and this may be ralated to the mechanism of anti-hypertrophic. Key Words Leonurus staehydrine; Myocardial cell hypertrophy; Myocardial hepertrophy; ROS; NF-κB

关 键 词：益母草水苏碱 心肌细胞 心肌肥大 ROS 核因子ΚB 

分 类 号：R285.5[医药卫生—中药学]