人工湿地不同植物根际微生物群落多样性比较研究  

Study on rhizosphere microbial community diversity of different plants grownin the constructed wetland

作　　者：武钰坤[1] 刘永军[1] 司英明[1] 熊家晴[1] 王晓昌[1] 

机构地区：[1]西安建筑科技大学,西北水资源与环境生态教育部重点实验室,西安710055

出　　处：《生态科学》2012年第3期318-323,共6页Ecological Science

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07317-004);陕西省重点实验室项目(2010JS026)

摘　　要：应用平板计数与PCR-DGGE技术相结合的方法,对人工湿地芦苇(Phragmites australis)、香蒲(Typha latifolia)和黑麦草(Lolium perenne)三种植物根际微生物群落多样性进行研究。结果表明,三种植物根际具有不同的微生物数量和群落结构;根际微生物数量显著高于非根际(P<0.05),两者具有不同的群落结构和优势种群;芦苇根际具有数目最多的常见物种和最大的物种均匀性,根际与根区多样性指数差别最大,根际效应R/S最大,表明芦苇能够更好的影响土壤微生物的富集和分布,提高微生物群落多样性。Rhizosphere microbial community diversity of different plants Phragmites australis, Typha latifolia, and Lolium perenne grown in constructed wetland was studied by plate count methods and PCR-DGGE. The results were as followed. Rhizosphere of different plants was different in community structure and microbial quantity. The microbial quantities in rhizosphere zone were significantly different from those in non-rhizosphere zone （P〈0.05）. Moreover, there were different community structure and dominant populations between rhizosphere and non-rhizosphere. Phragmites australis rhizosphere had the largest number of common species and species evenness. At the same time, it had the most significant rhizosphere effect （R/S）,The biggest different diversity index between rhizosphere and non-rhizosphere was also in P australis, which showed that it could better influence the enrichment and distribution of soil microbes, and improve the microbial communitiy diversity.

关 键 词：人工湿地 植物根际 群落多样性 PCR-DGGE 

分 类 号：X172[环境科学与工程—环境科学]