诱发大鼠肝癌早期病变中谷胱甘肽S-转移酶的研究  

STUDY ON GLUTATHIONE S—TRANSFERASE IN EARLY LESION OF INDUCED RAT HEPATOCARCINOMA

作　　者：林峰[1] 刘毅[1] 陈惠黎[1] 

机构地区：[1]上海医科大学生物化学教研室

出　　处：《肿瘤》1990年第5期214-216,共3页Tumor

摘　　要：谷胱甘肽S-转移酶(GST,E.C.2,5,1,18)是一类分布广泛的多功能蛋白,有多种同工酶形式,在大鼠中至少已发现12种,均为二聚体,分碱性、中性和酸性三大类。肝脏中GST的含量和种类最多,大多为碱性型。GST参与致癌剂的结合和解毒,又发现胎盘型GST(在大鼠中称GST-P或GST7-7,为胎盘中的唯一形式)能在早期肝癌中表达,是一个癌前病变的酶学标志。本文用Solt和Farber制订的顺序诱发大鼠肝癌,研究其早期病变中总GST活力及GST-P含量的变化,并用免疫组织化学方法观察了GST-P在肝组织中的定位。In the course of inducing hepatocarcinoma in rat. the total specific activity ofglutathione S-transferases (GST) in liver had a temporary increase in the Ist weekafter injection of diethylnitrosamine. After uptake of N-2-acetylaminofluorene in dietbeginning from the 3rd week and partial hepatectomy at the end of the 3rd week, theGST activity was significantly increased till the end of the 5th week. The.placenta formof rat GST (GST-P) in cytosol of rat liver was also determinod with sandwich ELTSAduring the malignant change by using Fab' fragment (prepared from rabbit anti-human GST-πIgG) conjugated with horseradish peroxidase (HRP). It was discoveredthat GST-P was increased remarkably 33 and 25 folds at the end of the 5th and 6th weekrespectively. Immunohistochemical stainittg using Fab'-ERP showe d that GST-P wasaccumulated in cell in hyperplastic.foci, and nuclei were positively stained earlier andstronger than the cytoplasm.

关 键 词：肝癌 谷胱甘肽 S-转移酶 大鼠 

分 类 号：R735.702[医药卫生—肿瘤]