原发性胃癌患者HLA Ⅱ类抗原DNA多态性的初步研究  

A PRELIMINARY STUDY OF HLA-CLASS Ⅱ GENE POLYMORPHISM IN PRIMARY GASTRIC CANCER PATIENTS

作　　者：陈诗书[1] 于丽莉[1] 赵涵芳[1] 孔良曼 林性玉 王亚新[1] 张来仪[1] 孙婕 何尧祥 薛建静 

机构地区：[1]上海第二医科大学生化教研组分子生物学实验室

出　　处：《肿瘤》1990年第6期259-260,245,共3页Tumor

摘　　要：有关胃癌与宿主的关系,特别是与机体免疫系统中主要组织相容性复合物(MHC)多态性的关系,至今未见有报道。担负机体特异免疫功能的淋巴细胞在识别异己抗原和引起免疫反应的过程均需要MHC基因产物的参加。因此分析胃癌患者MHC基因的多态性是研究胃癌与宿主关系的一个重要内容,有助于了解胃癌的发病机制和分子流行病学。Restriction fragment length polymorphisms (RFLPs) of DNA were studied withSoothern blot analysis in primary gastric cancer patients and normal individuals. UsingHLA-DQβ cDNA probe, the frequency of a 2.2 kb EcoRI band in gastric cancerpatients (0.78,n=40) is much higher than that in normal controls (0.27,n=60);the fre-quencies of the 3.6 kb and 3.0 kb BamHI bands in cancer group (0.75 and 0.50 respec-tively,n=54) are also higher than those in normal controls (0.40 and 0 respectively,n=25). The significance of these findings were discussed.

关 键 词：胃癌 HLAⅡ类抗原 DNA 多态性 

分 类 号：R735.203[医药卫生—肿瘤]