洋葱皮中黄酮类物质的提取及分离纯化研究  

Extraction,Separation and Purification of Flavones in Onionskin

作　　者：张仁堂[1] 秦静[2] 孙欣[1] 林云良[3] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]华中农业大学食品科学技术学院,武汉430070 [3]山东省分析测试中心,济南250014

出　　处：《中国食物与营养》2012年第8期50-54,共5页Food and Nutrition in China

基　　金：山东省高等学校科技计划项目(项目编号:J10LC14);泰安市大学生科技创新计划项目(项目编号:2009D1007)

摘　　要：采用超声波辅助法提取洋葱皮中黄酮类物质,通过柱层析等方法进行分离纯化,利用高效液相色谱对黄酮类物质进行检测,在超声波辅助萃取洋葱皮中总黄酮的研究中,确定乙醇浓度80%、料液比1∶10、超声时间20min条件下对总黄酮提取率最高。获得总黄酮物质后采用萃取、聚酰胺柱层析进行分离纯化,获得不同浓度乙醇洗脱组分,建立HPLC方法为进样量5μL、检测波长360nm、甲醇∶水(含0.1%冰乙酸)=45∶55、柱温为室温。分离纯化中得到结晶,通过质谱、核磁共振鉴定此晶体物质为槲皮素。This paper studied the technological process of extracting flavones from onionskin, established a HPLC method for determining the flavones in the extraction of the onionskin. The optimum ultrasonic extraction conditions for flavones were also established which were soak onion for 20min with 80% ethanol concentration and ratio of liquid to solid was l：10. In the study of the HPLC method for determining the flavones, we got the condition of a 5k^L sample injected, detection was carried out at 360 nm, a mixture of methanol and water with 0. 1% glacial acetic acid （45：55） was used as mobile phase. In the course of separation and purification, we got the crystallization with high pure. By the way of MS and NMR, we knew this material was quercetin.

关 键 词：洋葱皮 黄酮 聚酰胺柱层析 HPLC 槲皮素 

分 类 号：TQ914.1[化学工程]