SSR分子标记分析彩色马铃薯品种间的遗传关系  

Genetic diversity research of color potato cultivars by SSR molecular markers

作　　者：李先平[1,2,3] 王冬冬 陈秀华[1,2] 朱延明[1] 陈勤[1,2] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]加拿大农业及农业食品部莱斯布里奇研究中心 [3]云南省农业科学院经济作物研究所,昆明650205

出　　处：《东北农业大学学报》2012年第7期61-69,共9页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(30860153);国家留学基金项目(留金法[2009]5004号);云南省马铃薯工程技术研究中心项目(2011DH018)

摘　　要：利用SSR分子标记对来源不同的30份彩色马铃薯种质材料进行遗传多样性研究。结果表明,41个SSR引物扩增30个供试品种得到152条带,其中128个为多态性带,平均每个引物扩增3.12个多态性条带,其中包括一些品种特异性的SSR位点。UPGMA法进行聚类分析,在简单遗传相似系数SM=0.65处可将供试材料分成两大类群:类群Ⅰ为来自北美和欧洲的彩色马铃薯品种,在SM=0.72处可分为紫色马铃薯亚群,红色马铃薯亚群和白色马铃薯亚群;类群Ⅱ主要是不同来源的彩色马铃薯种质资源,包括二倍体原始栽培种,二倍体野生种和四倍体栽培种安弟斯亚种资源。聚类遗传关系表明类群Ⅰ和类群Ⅱ遗传关系较远,性状差异较大,可以作为极好的遗传资源进行杂交育种,扩展和丰富彩色马铃薯种质遗传背景。The genetic diversity among 30 pigmented potatoes were investigated by SSR（simple sequence repeats） molecular markers.The results showed that 152 bands were amplified and 128 were showed polymorphic using 41 SSR primer pairs.The average number of alleles per SSR locus was 3.12.Several accessions and group specific alleles were detected.The UPGMA（unweighted pair group method arithmetic averages） analysis based on SSR data showed that all the accessions could be classified into two groups when simple matching coefficient（SM） equal to 0.65.Group I included most of the color potato cultivars from North America and Europe,which could be divided to purple potato subgroup,red potato subgroup and white potato subgroup.There were many kinds of color potato germplasms in Group II,which included diploid primary cultivar,diploid wild specials and tetraploid color resources.The cluster results showed that there were far genetic relationship and many differences between Group I and Group II.Using the two groups to make cross each other could create new pigmented resources for color potato breeding.

关 键 词：SSR 彩色马铃薯 遗传多样性 

分 类 号：S532[农业科学—作物学]