人淋巴瘤细胞株OCI-Ly19生物学特性研究和小鼠模型建立  

Human diffuse large B-cell lymphoma cell line OCI-Ly19: Biological characteristics and establishment of a mouse model

作　　者：南英[1] 韩香萍[2] 路玲玲[1] 向荣[1] 汪洋[1] 

机构地区：[1]南开大学医学院分子病毒和病毒免疫实验室,天津医学硕士研究生300071 [2]南开大学生命科学学院生物化学和分子生物学系

出　　处：《医学研究生学报》2012年第8期793-797,共5页Journal of Medical Postgraduates

基　　金：天津市科委中国瑞典合作重大项目(09ZCZDSF04000)

摘　　要：目的弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是成人淋巴瘤中最常见的病理类型。DLBCL分型对指导疾病的治疗和预后有较大的意义,但各型DLBCL的发病机制及治疗、预后尚还有待深入研究。文中探索DLBCL细胞株OCI-Ly19的体外培养条件和生物学特性,肿瘤相关分子的表达及DLBCL动物模型的建立方法。方法观察OCI-Ly19细胞生长形态及生物学特性,分析比较不同实验条件对细胞生长的影响,用流式细胞术分析相关抗原表达,将OCI-Ly19细胞皮下接种SCID小鼠,观察肿瘤生长状况及组织学形态。结果 OCI-Ly19细胞在适当培养条件下生长良好,多数重要的B细胞和肿瘤相关标记物均有不同程度的表达;未经照射的SCID小鼠皮下接种107个OCI-Ly19细胞,成瘤率75%,组织学特征符合人类DLBCL的特点。结论获得了OCI-Ly19细胞株体外培养的最佳条件及相关免疫标志物表达情况。成功构建了人DLBCL的小鼠模型,为进一步进行相关研究提供了实验基础。Objective Diffuse large B-cell lymphoma （DLBCL） is a most common pathological type of lymphoma in adults. The typing of DLBCL helps a lot the treatment and prognosis of the disease, and deeper studies are needed on the pathogenesis, management and prognosis of different types of DLBCL. The aim of this study was to investigate the in vitro culture conditions and biological characteristics of OCI-Ly19 cells of human DLBCL, the expression of tumor-associated molecules and the establishment of an animal model of human DLBCL. Methods We observed the histological and biological characteristics of OCI-Lyl9 cells under different cul- ture conditions, analyzed the expressions of B-cells and tumor-related markers by flow eytometry, and observed the formation and his-tology of tumors following subcutaneous injection of OCI-Ly19 cells into SCID mice. Results OCI-Lyl9 cells grew well under appro- priate culture conditions. Flow cytometry showed high expressions of B-cells and tumor-related markers （50% -98% ） and median expressions of proliferation- and apoptosis-related markers （20% -53% ） of the cultured OCI-Lyl9 cells. Subcutaneous injection of 107 OCI-Ly19 cells into the SCID mice produced 75% of tumor formation with the characteristics of human DLBCL. Conclusion We obtained most appropriate conditions for the in vitro culture of OCI-Ly19 cells and the expressions of relevant immune markers, and successfully established a mouse model of human DLBCL.

关 键 词：弥漫大B细胞淋巴瘤 动物模型 细胞株 OCI—Ly19 

分 类 号：R733[医药卫生—肿瘤]