小鼠肺腺癌模型的建立及肿瘤病理分析  

Establishment and pathological characterization of a mouse model of lung adenocarcinoma

作　　者：侯敏[1] 戴丽军[2] 谭小军[3] 黄月玲[2] 高蔚樱[3] 吕嘉春[4] 任韵[1] 周敏仪[1] 朴金松[3] 劳淑贞[3] 梁成结[2] 冯瑗瑜[2] 

机构地区：[1]广州医学院附属肿瘤医院肿瘤研究所,广州510095 [2]广州医学院实验动物中心,广州510182 [3]广州医学院附属肿瘤医院病理科,广州510095 [4]广州医学院公共卫生学院,呼吸疾病国家重点实验室,广州510182

出　　处：《中国实验动物学报》2012年第4期75-79,F0003,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：广州医学院博士启动基金(编号:2010C32);广州医学院附属肿瘤医院重大专项基金(编号:2010-yz-02)

摘　　要：目的用乙基亚硝脲(ENU)在BABL/c小鼠建立肺腺癌模型并对ENU所诱发的肺腺癌进行病理观察。方法妊娠17d的SPF级母鼠腹腔接受ENU或缓冲液注射,在子代鼠的鼠龄满32周时收获其全肺标本,对肺组织进行常规石蜡半连续切片,HE染色,镜下观察肿瘤病理。结果 ENU经胎盘一次性诱发子代鼠多发性肺肿瘤形成,病理显示这些肿瘤为处于不同发展阶段的腺瘤和腺癌,腺癌的类型有细支气管肺泡癌样腺癌(雌性:5/6,雄性:4/6)和分化不等的腺癌(雌性:4/6,雄性:5/6),诱癌频率在雌、雄性小鼠均为5/6,癌变频率在雌性16/43,雄性12/31。结论成功建立了小鼠肺腺癌模型,肿瘤病理的多样性提示癌变机制在分子水平的复杂性。Objective To establish a mouse model of lung adenocarcinoma induced by N-ethyl-N-nitrosourea（ENU） in mice and to characterize its tumor pathology.Methods BABL/c mice were used to establish the lung cancer model.On the 17th day of pregnancy,the parental female mice received intraperitoneally ENU or buffer injection,respectively.The 32-week old offsprings were sacrificed and entire lungs were harvested and processed for histopathological observation of HE stained semiserial sections.The pathological features of the lung tumors were assessed.Results Multiple tumors in offspring’s lungs were induced transplacentally by a single injection of ENU.The tumors were at different development stages of adenoma or adenocarcinoma.The adenocarcinomas were classified as bronchioloalveolar-like adenocarcinoma（female： 5/6;male： 4/6） and adenocarcinoma of various differentiation（female： 4/6;male： 5/6）.The frequency of cancer development was 5 out of 6 mice in both sexes.The frequency of tumors which developed into malignant tumors was 16 out of 43 tumors in female and 12 out of 31 tumors in male mice.Conclusions We have successfully established a mouse model of lung adenocarcinoma in mouse offsprings by a single injection of ENU in pregnant female mice.The heterogeneous presentation of tumor pathology indicates the complex molecular mechanisms of its carcinogenesis.

关 键 词：肺 腺癌 腺瘤 乙基亚硝脲 

分 类 号：R73-34[医药卫生—肿瘤]